Coyo Natàlia, Leiva Marta, Costa Daniel, Molina Rafael, Nicolás Olga, Ríos Jose, Peña Maria Teresa
Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Servei d'Oftalmologia, Fundació Hospital Clínic Veterinari, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Vet Ophthalmol. 2019 Mar;22(2):177-182. doi: 10.1111/vop.12578. Epub 2018 May 15.
To determine endothelial cell density (ECD) and morphology and morphometry of corneal endothelial cells in the tawny owl (Strix aluco), as well as to report the effects of aging on these parameters.
Twenty tawny owls were included in the study and classified into 2 groups according to their age: fledglings (<1 year old) and adults (>1 year old). Central corneal endothelium was studied by means of noncontact specular microscopy (Specular Microscope SP-2000P; Topcon, Tokyo, Japan), and results for ECD (cells/mm ), mean cell area ((MCA (μm )), polymegathism (CV), and pleomorphism (% hexagonal cells) were obtained. Results are described by median, interquartile range (25th, 75th percentiles), and absolute range for ECD, MCA, pleomorphism, and polymegathism. In addition, inferential analyses by Mann-Whitney U test were also performed. A two-tailed Type I error of 5% was established.
Results in fledglings were as follows: ECD = 2864 cells/mm , MCA = 348 μm , % hexagonal cells = 72.75%, and CV = 21. Results in adults were as follows: ECD = 2602 cells/mm , MCA = 384 μm , % hexagonal cells = 78.83%, and CV = 16. No significant differences in ECD and MCA were seen between the groups (P > .05), although there were significant differences in % hexagonal cells and CV (P < .05).
Tawny owls present a uniform endothelium in cell size and shape, although ECD and MCA differ greatly from other bird species. Differences in ECD and MCA could not be found between fledglings and adults probably because of the youth of adult specimens, although there were differences in pleomorphism and polymegathism.
确定仓鸮（Strix aluco）角膜内皮细胞密度（ECD）、形态及形态测量数据，并报告衰老对这些参数的影响。
20只仓鸮纳入本研究，根据年龄分为两组：雏鸟（<1岁）和成年鸟（>1岁）。采用非接触式角膜内皮显微镜（Specular Microscope SP - 2000P；日本东京拓普康公司）研究中央角膜内皮，获得ECD（细胞/mm²）、平均细胞面积（MCA，μm²）、细胞大小不均一性（CV）和多形性（六边形细胞百分比）的结果。结果以ECD、MCA、多形性和细胞大小不均一性的中位数、四分位数间距（第25、75百分位数）和绝对范围描述。此外，还采用曼-惠特尼U检验进行推断性分析。设定双侧I型错误率为5%。
雏鸟的结果如下：ECD = 2864个细胞/mm²，MCA = 348μm²，六边形细胞百分比 = 72.75%，CV = 21。成年鸟的结果如下：ECD = 2602个细胞/mm²，MCA = 384μm²，六边形细胞百分比 = 78.83%，CV = 16。两组间ECD和MCA无显著差异（P > 0.05），但六边形细胞百分比和CV有显著差异（P < 0.05）。
仓鸮的内皮细胞在大小和形状上呈现出一致性，尽管ECD和MCA与其他鸟类有很大差异。雏鸟和成年鸟之间未发现ECD和MCA的差异，可能是因为成年样本相对年轻，不过在多形性和细胞大小不均一性方面存在差异。
