Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan.
Kang Jian Biotech Co., Ltd., Nantou, Taiwan.
Int J Biol Macromol. 2018 Sep;116:728-734. doi: 10.1016/j.ijbiomac.2018.05.047. Epub 2018 May 12.
Polysaccharides including β-glucans are important bioactive components of mushroom. Xylaria nigripes is a popular medicinal fungus that has been used for treating trauma, insomnia and mental illness. This study examined the physicochemical characteristics and anti-inflammatory activities of water soluble non-digestible polysaccharides (TXNP and CXNP) from fruiting bodies of two cultivated X. nigripes strains (TXN and CXN). Results showed that both TXNP and CXNP possessed relatively similar FT-IR spectra. TXNP had a triple helix conformation and molecular weight of 853.8 kDa, whereas the molecular weight of CXNP was 14.7 kDa. The monosaccharide composition of TXNP was predominantly glucose, whereas CXNP contained xylose, mannose and glucose. Although both TXNP and CXNP dose-dependently suppressed the production of NO, IL-1β, TNF-α and PGE2, as well as the expression of iNOS, COX-2 and NF-κB in the lipopolysaccharide-induced RAW264.7 macrophages, the potency of TXNP was stronger. This study reveals that under similar conditions of cultivation and extraction procedures, the different physicochemical characteristics of polysaccharides from TXN and CXN may have contributed to the differences in their anti-inflammatory potency.
多糖包括β-葡聚糖是蘑菇的重要生物活性成分。炭角菌是一种受欢迎的药用真菌，用于治疗创伤、失眠和精神疾病。本研究检测了两种栽培的黑炭角菌菌株（TXN 和 CXN）子实体中水溶性不可消化多糖（TXNP 和 CXNP）的理化特性和抗炎活性。结果表明，TXNP 和 CXNP 具有相似的傅里叶变换红外光谱（FT-IR）。TXNP 具有三螺旋构象和 853.8 kDa 的分子量，而 CXNP 的分子量为 14.7 kDa。TXNP 的单糖组成为葡萄糖，而 CXNP 含有木糖、甘露糖和葡萄糖。尽管 TXNP 和 CXNP 均呈剂量依赖性抑制脂多糖诱导的 RAW264.7 巨噬细胞中 NO、IL-1β、TNF-α 和 PGE2 的产生以及 iNOS、COX-2 和 NF-κB 的表达，但 TXNP 的活性更强。本研究表明，在相似的培养和提取条件下，TXN 和 CXN 多糖的不同理化特性可能导致其抗炎活性的差异。
