Hu Dongbao, Li Ming
College of Resource and Environment, Yuxi Normal University, Yuxi, 653100, P. R. China.
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Lanhei Road 132, Kunming, 650201, P. R. China.
Chem Biodivers. 2017 Jan;14(1). doi: 10.1002/cbdv.201600173. Epub 2016 Dec 19.
Three new ergot alkaloids, xylanigripones A - C (1 - 3) together with three known compounds, agroclavine (4), 8,9-didehydro-10-hydroxy-6,8-dimethylergolin (5), and (6S)-agroclavine N-oxide (6) were isolated from the fungus Xylaria nigripes (Kl.) Sacc. Their structures were elucidated by comprehensive spectroscopic analyses and high-resolution mass spectrometry as well as by comparison with the literature. The absolute configuration was determined by Density Functional Theory (DFT) calculation methods. In addition, all of the compounds were evaluated for bioactivity via a cytotoxicity assay, an acetylcholinesterase inhibition assay and a cholesterol ester transfer protein inhibition assay.
从真菌黑柄炭角菌（Xylaria nigripes (Kl.) Sacc.）中分离出三种新的麦角生物碱，即木聚糖黑柄菌素A - C（1 - 3），以及三种已知化合物，即农吉利麦角碱（4）、8,9 - 二脱氢 - 10 - 羟基 - 6,8 - 二甲基麦角灵（5）和（6S） - 农吉利麦角碱N - 氧化物（6）。通过综合光谱分析、高分辨率质谱以及与文献对比对它们的结构进行了阐明。通过密度泛函理论（DFT）计算方法确定了绝对构型。此外，通过细胞毒性试验、乙酰胆碱酯酶抑制试验和胆固醇酯转移蛋白抑制试验对所有化合物的生物活性进行了评估。
