Organic Nanoelectronics Laboratory and KNU Institute for Nanophotonics Applications (KINPA), Department of Chemical Engineering, School of Applied Chemical Engineering, Kyungpook National University, Daegu 41566, Republic of Korea.
Laboratory of Transmission, Control and Immunobiology of Infections (LTCII), Institut Pasteur de Tunis, LR11IPT02, Tunis-Belvédère 1002, and Université Tunis El Manar, Tunis 1068, Tunisia.
J Hazard Mater. 2018 Aug 5;355:17-24. doi: 10.1016/j.jhazmat.2018.04.063. Epub 2018 Apr 27.
Here we report that superoxide, one of the hazardous reactive oxygen species (ROS), can be quickly detected by flexible organic field-effect transistors (OFETs) with the polyphenol-embedded conjugated polymer micro-channels. Rutin, one of the abundant polyphenols found in a variety of plants, was employed as a sensing molecule and embedded in the poly(3-hexylthiophene) (P3HT) matrix. The rutin-embedded P3HT layers showed randomly distributed micro-domains, which became bigger as the rutin content increased. The best transistor performance was achieved at the rutin content of 10 wt%, while the OFETs exhibited proper and controllable transistor performances even in the phosphate buffer solutions. The sensing test revealed that the present OFET sensors could stably detect superoxide using very small amount (<10 μl) of samples at extremely low concentrations (500 pM), while they exhibited outstanding stability and durability upon repeated detection and storage-reuse tests. Finally, the present flexible OFET sensors could deliver confident sensing results for the detection of superoxide generated from the mouse RAW264.7 macrophages.
在这里，我们报告超氧阴离子，一种有害的活性氧（ROS），可以通过嵌入多酚的共轭聚合物微通道的柔性有机场效应晶体管（OFET）快速检测。芦丁是多种植物中丰富的多酚之一，被用作传感分子并嵌入聚（3-己基噻吩）（P3HT）基质中。嵌入芦丁的 P3HT 层呈现出随机分布的微区，随着芦丁含量的增加而变大。在芦丁含量为 10wt%时，晶体管性能最佳，而即使在磷酸盐缓冲溶液中，OFET 也表现出适当且可控制的晶体管性能。传感测试表明，本 OFET 传感器可以使用非常少量（<10μl）的样品在极低浓度（500pM）下稳定地检测超氧阴离子，并且在重复检测和存储-再利用测试中表现出出色的稳定性和耐用性。最后，本柔性 OFET 传感器可以为检测来自小鼠 RAW264.7 巨噬细胞的超氧阴离子提供可靠的传感结果。
