Amaral C C F, Ormiga F, Boldrini L C, Miranda P G, Mendonça T A, Granjeiro J M, Gomes J A C P
Department of Metallurgy and Materials, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Department of Dental Clinic, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Int Endod J. 2018 Dec;51(12):1434-1445. doi: 10.1111/iej.12950. Epub 2018 Jun 12.
To evaluate the effects of the [NaF 12 g L + NaCl 1 g L ] solution used in the electrochemical dissolution process of fractured endodontic files, as well as its NiTi-containing product, on dentine hardness, topography and human fibroblast viability.
Sixty single-rooted human teeth were evaluated for dentine microhardness using the Vickers hardness test and the area and number of dentinal tubules by scanning electron microscopy. The samples were divided according to the dentine surface treatment: distilled water; 17% EDTA; [NaF 12 g L + NaCl 1 g L ]; and 17% EDTA + [NaF 12 g L + NaCl 1 g L ]. Thirty-six single-rooted human teeth were divided according to the irrigation protocol: Dulbecco's Modified Eagle's Medium + 10% foetal bovine serum; 5.25% NaOCl; [NaF 12 g L + NaCl 1 g L ]; and [NaF 12 g L + NaCl 1 g L + NiTi]. The extracts in contact with the apical foramen were used in the MTT assay to evaluate human fibroblast viability, with dilutions of 100%, 50%, 25% and 12.5%. Statistical tests used were paired t-tests, one-way anova, Tukey's test, Kruskal-Wallis test and Dunn's post-test.
The [NaF 12 g L + NaCl 1 g L ] solution did not modify dentine microhardness or the average dentinal tubule area. However, EDTA induced changes in dentine structure and microhardness (P < 0.05). The [NaF 12 g L + NaCl 1 g L ] solution, and its NiTi-containing product had lower cytotoxicity than NaOCl at dilutions of 25% and 50% (P < 0.01).
The [NaF 12 g L + NaCl 1 g L ] solution did not alter dentine microhardness or damage the dentine structure. It also demonstrated lower cytotoxicity than NaOCl.
评估用于折断根管锉电化学溶解过程的[氟化钠12 g/L +氯化钠1 g/L]溶液及其含镍钛的产物对牙本质硬度、表面形貌和人成纤维细胞活力的影响。
使用维氏硬度测试评估60颗单根人牙的牙本质显微硬度，并用扫描电子显微镜观察牙本质小管的面积和数量。根据牙本质表面处理方式将样本分组：蒸馏水；17%乙二胺四乙酸（EDTA）；[氟化钠12 g/L +氯化钠1 g/L]；以及17% EDTA + [氟化钠12 g/L +氯化钠1 g/L]。根据冲洗方案将36颗单根人牙分组：杜尔贝科改良伊格尔培养基+10%胎牛血清；5.25%次氯酸钠（NaOCl）；[氟化钠12 g/L +氯化钠1 g/L]；以及[氟化钠12 g/L +氯化钠1 g/L +镍钛]。将与根尖孔接触的提取物用于MTT法评估人成纤维细胞活力，稀释度分别为100%、50%、25%和12.5%。使用的统计检验方法包括配对t检验、单因素方差分析、图基检验、克鲁斯卡尔-沃利斯检验和邓恩事后检验。
[氟化钠12 g/L +氯化钠1 g/L]溶液未改变牙本质显微硬度或牙本质小管的平均面积。然而，EDTA引起了牙本质结构和显微硬度的变化（P < 0.05）。在25%和50%稀释度下，[氟化钠12 g/L +氯化钠1 g/L]溶液及其含镍钛的产物的细胞毒性低于NaOCl（P < 0.01）。
[氟化钠12 g/L +氯化钠1 g/L]溶液未改变牙本质显微硬度，也未损害牙本质结构。它还显示出比NaOCl更低的细胞毒性。
