[大黄对高氧诱导的新生大鼠支气管肺发育不良的影响]
[Effect of rhubarb on neonatal rats with bronchopulmonary dysplasia induced by hyperoxia].
作者信息
Yin Ling-Ling, Ye Zhen-Zhi, Tang Li-Jun, Guo Liang, Huang Wei-Min
机构信息
Department of Neonatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
出版信息
Zhongguo Dang Dai Er Ke Za Zhi. 2018 May;20(5):410-415. doi: 10.7499/j.issn.1008-8830.2018.05.014.
OBJECTIVE
To study the effect of rhubarb on neonatal rats with bronchopulmonary dysplasia (BPD) induced by hyperoxia.
METHODS
A total of 64 rats (postnatal day 4) were randomly divided into four groups: air control, rhubarb control, hyperoxia model, and hyperoxia+rhubarb (n=16 each). The rats in the hyperoxia model and hyperoxia+rhubarb groups were exposed to hyperoxia (60% O2) to establish a BPD model. The rats in the rhubarb control and hyperoxia+rhubarb groups were given rhubarb extract suspension (600 mg/kg) by gavage daily. The pathological changes of lung tissue were evaluated by hematoxylin-eosin staining on postnatal days 14 and 21. The content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) were measured by spectrophotometry. The mRNA and protein expression levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were determined by RT-PCR and Western blot respectively.
RESULTS
The hyperoxia model group showed reduced alveolar number, increased alveolar volume, and simplified alveolar structure, which worsened over the time of exposure to hyperoxia. These pathological changes were significantly reduced in the hyperoxia+rhubarb group. On postnatal days 14 and 21, compared with the air control and rhubarb control groups, the hyperoxia model group had significantly reduced radical alveolar count (RAC), significantly reduced activity of SOD in the lung tissue, and significantly increased content of MDA and mRNA and protein expression levels of TNF-α and IL-6 (P<0.05). Compared with the hyperoxia model group, the hyperoxia+rhubarb group had significantly increased RAC, significantly increased activity of SOD in the lung tissue, and significantly reduced content of MDA and mRNA and protein expression levels of TNF-α and IL-6 (P<0.05).
CONCLUSIONS
Rhubarb may play a protective role in rats with BPD induced by hyperoxia through inhibiting inflammatory response and oxidative stress.
目的
研究大黄对高氧诱导的新生大鼠支气管肺发育不良（BPD）的影响。
方法
将64只出生4天的大鼠随机分为四组：空气对照组、大黄对照组、高氧模型组和高氧+大黄组（每组n = 16）。高氧模型组和高氧+大黄组的大鼠暴露于高氧（60% O₂）以建立BPD模型。大黄对照组和高氧+大黄组的大鼠每天经口灌胃给予大黄提取物混悬液（600 mg/kg）。在出生后第14天和第21天，通过苏木精-伊红染色评估肺组织的病理变化。采用分光光度法测定丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性。分别通过逆转录-聚合酶链反应（RT-PCR）和蛋白质免疫印迹法（Western blot）测定肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的mRNA和蛋白表达水平。
结果
高氧模型组肺泡数量减少，肺泡体积增大，肺泡结构简化，且随着高氧暴露时间的延长而加重。高氧+大黄组的这些病理变化明显减轻。在出生后第14天和第21天，与空气对照组和大黄对照组相比，高氧模型组的肺泡计数（RAC）显著降低，肺组织中SOD活性显著降低，MDA含量以及TNF-α和IL-6的mRNA和蛋白表达水平显著升高（P<0.05）。与高氧模型组相比，高氧+大黄组的RAC显著增加，肺组织中SOD活性显著增加，MDA含量以及TNF-α和IL-6的mRNA和蛋白表达水平显著降低（P<0.05）。
结论
大黄可能通过抑制炎症反应和氧化应激对高氧诱导的BPD大鼠发挥保护作用。
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