Zhang Hang, Li Jingjing, Cao Yanan, Dong Xiang, Gao Cong, Li Fanfan
Department of Oncology, the Second Affiliated Hospital of Anhui Medical University, Hefei 230601, China.
Zhongguo Fei Ai Za Zhi. 2018 May 20;21(5):351-357. doi: 10.3779/j.issn.1009-3419.2018.05.01.
Adipocytes in the tumor microenvironment may provide the metabolic fuel or signal transduction through media and other means to promote a variety of malignant proliferation and invasion, of tumor cells, but their role in lung cancer progression is still unclear. The purpose of this study was to investigate the effect of adipocytes on lung cancer cell biology.
3T3-L1 pre-adipocytes were induced into mature adipocytes. The cell morphology was observed by microscopy and Oil Red O staining. MTT assay, colony formation assay, wound-healing and Transwell methods were used to detect lung cancer cell proliferation, migration and invasion ability. The content of triglyceride in cells was determined by colorimetry.
The morphology of lung adenocarcinoma A549 cells became more slender after co-culture with mature adipocytes, and the proliferation and cloning ability were significantly enhanced (P<0.05). In addition, mature adipocytes can also promote the migration ability (P<0.05), invasion ability (P<0.01) and accumulation of intracellular lipid (P<0.05) of A549 cells.
These findings suggested that adipocytes in tumor microenvironment can promote the proliferation, migration and invasion of lung adenocarcinoma A549 cells, which may be related to lipid metabolism.
肿瘤微环境中的脂肪细胞可能通过介质等提供代谢燃料或信号转导，以促进肿瘤细胞的多种恶性增殖和侵袭，但其在肺癌进展中的作用仍不清楚。本研究旨在探讨脂肪细胞对肺癌细胞生物学的影响。
将3T3-L1前脂肪细胞诱导为成熟脂肪细胞。通过显微镜观察和油红O染色观察细胞形态。采用MTT法、集落形成法、伤口愈合法和Transwell法检测肺癌细胞的增殖、迁移和侵袭能力。用比色法测定细胞内甘油三酯含量。
肺癌腺癌A549细胞与成熟脂肪细胞共培养后形态变得更加细长，增殖和克隆能力显著增强（P<0.05）。此外，成熟脂肪细胞还可促进A549细胞的迁移能力（P<0.05）、侵袭能力（P<0.01）及细胞内脂质蓄积（P<0.05）。
这些发现提示肿瘤微环境中的脂肪细胞可促进肺癌腺癌A549细胞的增殖、迁移和侵袭，这可能与脂质代谢有关。
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