Sigmovir Biosystems Inc., 9610 Medical Center Drive, Suite 100, Rockville, MD, 20850, USA.
Department of Microbiology and Physiological Systems, Program of Immunology and Microbiology, University of Massachusetts Medical School, Worcester, MA, 01655, USA.
Nat Commun. 2018 May 15;9(1):1904. doi: 10.1038/s41467-018-04216-6.
Respiratory syncytial virus (RSV) is the most common cause of bronchiolitis in infants. Maternal immunization is an option to increase maternal antibody levels and protect infants from infection. Here we assess the efficacy of virus-like particle (VLP) vaccine candidates containing stabilized pre-fusion (pre-F) or post-fusion (post-F) conformations of the RSV F protein and the attachment RSV G protein in a maternal immunization model using cotton rats. VLP vaccines containing RSV F and G proteins strongly boost pre-existing RSV immunity in dams preventing their perinatal drop in immunity. Boosting is stronger for the pre-F VLP than for the post-F VLP or purified subunit F protein vaccines, giving an advantage on mothers' protection. VLP immunization of dams provides significant protection to pups from RSV challenge and reduced pulmonary inflammation. Collectively, our results show that a VLP vaccine with RSV F and G proteins is safe and effective for maternal and adult vaccination.
呼吸道合胞病毒（RSV）是婴儿毛细支气管炎最常见的病因。母体免疫接种是增加母体抗体水平并保护婴儿免受感染的一种选择。在这里，我们使用棉鼠评估了含有 RSV F 蛋白稳定的预融合（pre-F）或融合后（post-F）构象以及 RSV G 蛋白的病毒样颗粒（VLP）候选疫苗在母体免疫接种模型中的功效。含有 RSV F 和 G 蛋白的 VLP 疫苗可强烈增强母体中现有的 RSV 免疫力，防止其围产期免疫力下降。与 post-F VLP 或纯化亚单位 F 蛋白疫苗相比，pre-F VLP 的增强作用更强，这为母亲提供了保护优势。VLP 免疫接种可显著降低 RSV 对幼仔的挑战，并减少肺部炎症。总的来说，我们的结果表明，含有 RSV F 和 G 蛋白的 VLP 疫苗对母体和成人疫苗接种是安全有效的。
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