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白藜芦醇通过抑制细胞凋亡和脂质过氧化显著提高了牛性别分选精液的受精能力。

Resveratrol significantly improves the fertilisation capacity of bovine sex-sorted semen by inhibiting apoptosis and lipid peroxidation.

机构信息

Embryo Biotechnology and Reproduction Laboratory and the Centre of Domestic Animal Reproduction & Breeding, Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan Western Road, Haidian District, Beijing, 100193, P.R. China.

Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, No. 159 North of Industry Road, Jinan, Shandong, 250131, P.R. China.

出版信息

Sci Rep. 2018 May 15;8(1):7603. doi: 10.1038/s41598-018-25687-z.

DOI:10.1038/s41598-018-25687-z
PMID:29765076
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5954094/
Abstract

The aim of this study was to test the effects of five different concentrations (0, 10, 10, 10, and 10 M) of resveratrol (Res) supplementation in bull sperm washing and fertilisation medium on levels of reactive oxygen species (ROS), phosphatidylserine (PS) externalisation, mitochondrial membrane potential (Δψm), ATP and malondialdehyde (MDA), acrosomal integrity, blastocyst rate, and blastocyst quality after in vitro fertilisation (IVF). The results for sex-sorted sperm from three bulls showed: (1) ROS and MDA levels in 10 M and 10 M Res groups were significantly lower than those of controls (P < 0.05); (2) the percentage of viable sperm, percentage of sperm with high Δψm, and the ATP content in 10 M and 10 M Res groups were significantly higher than those of controls (P < 0.05); (3) the percentage of viable sperm with acrosomal integrity, and the blastocyst percentage and quality of the 10 M Res group were significantly higher than those of controls (P < 0.05). In conclusion, 10 M Res supplementation in washing and fertilisation medium of sex-sorted bull sperm significantly decreased ROS, PS externalisation, and MDA, and protected mitochondrial function and acrosomal integrity, thereby increasing blastocyst percentage and quality following IVF.

摘要

本研究旨在测试五种不同浓度（0、10、10、10 和 10 M）白藜芦醇（Res）在公牛精子洗涤和受精液中的添加对活性氧（ROS）、磷脂酰丝氨酸（PS）外翻、线粒体膜电位（Δψm）、ATP 和丙二醛（MDA）、顶体完整性、体外受精（IVF）后囊胚率和囊胚质量的影响。来自三头公牛的分选精子的结果表明：（1）10 M 和 10 M Res 组的 ROS 和 MDA 水平明显低于对照组（P < 0.05）；（2）10 M 和 10 M Res 组的活精子百分比、高 Δψm 精子百分比和 ATP 含量显著高于对照组（P < 0.05）；（3）10 M Res 组具有顶体完整性的活精子百分比以及囊胚率和质量均显著高于对照组（P < 0.05）。综上所述，10 M Res 在分选公牛精子的洗涤和受精液中的添加可显著降低 ROS、PS 外翻和 MDA，并保护线粒体功能和顶体完整性，从而提高 IVF 后的囊胚率和质量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc26/5954094/dd17bd249a0c/41598_2018_25687_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc26/5954094/499cf3f093a9/41598_2018_25687_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc26/5954094/3ca01e24b58b/41598_2018_25687_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc26/5954094/4d50d895e5c5/41598_2018_25687_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc26/5954094/13493efef9c4/41598_2018_25687_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc26/5954094/f4c72fccd0bd/41598_2018_25687_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc26/5954094/0246c81b63c0/41598_2018_25687_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc26/5954094/7d1539f05af6/41598_2018_25687_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc26/5954094/7d9d7da923f2/41598_2018_25687_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc26/5954094/dd17bd249a0c/41598_2018_25687_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc26/5954094/499cf3f093a9/41598_2018_25687_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc26/5954094/3ca01e24b58b/41598_2018_25687_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc26/5954094/4d50d895e5c5/41598_2018_25687_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc26/5954094/13493efef9c4/41598_2018_25687_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc26/5954094/f4c72fccd0bd/41598_2018_25687_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc26/5954094/0246c81b63c0/41598_2018_25687_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc26/5954094/7d1539f05af6/41598_2018_25687_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc26/5954094/7d9d7da923f2/41598_2018_25687_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc26/5954094/dd17bd249a0c/41598_2018_25687_Fig9_HTML.jpg

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