2016年在马西·达内什瓦里医院重症监护病房收治的呼吸机相关性肺炎患者中对PIKR和ATpA基因表达的研究。
Investigating PIKR and ATpA Genes Expressions in Ventilator-Associated Pneumonia Patients Admitted to the Intensive Care Unit of Masih Daneshvari Hospital in 2016.
作者信息
Jamaati Hamidreza, Bahrami Naghmeh, Daustany Mahya, Tabarsi Payam, Farzanegan Behrooz, Hashemian Seyed Mohammadreza, Mohamadnia Abdolreza
机构信息
Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Craniomaxillofacial Research center, Tehran University of Medical Sciences, Tehran, Iran. Oral and Maxillofacial Surgery Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
Rep Biochem Mol Biol. 2018 Apr;6(2):118-124.
BACKGROUND
Infectious diseases such as ventilator- associated pneumonia (VAP) are one of the serious problems in intensive care units (ICU) of hospitals. To date, there has been no appropriate clinical and diagnostic marker for early detection of this disease. In this study, expression of PIKR and ATpA genes in patients with VAP were assessed to be used as biomarkers to identify and confirm the disease.
METHODS
This study was conducted by using peripheral blood samples of 60 individuals, including 30 patients with VAP and 30 healthy volunteers. First, the peripheral blood samples were taken and then RNA was extracted and converted into cDNA. Finally, the assessment of genes was performed by Real-time PCR.
RESULTS
In peripheral blood samples, 46.6% and 30% were positive for PIKR expression in patients and healthy groups, respectively. The ATpA expression in patients and healthy controls were found 40% and 23.3%, respectively. Comparing the ΔCT obtained for the PIKR and ATpA genes showed statistically significant differences between the two groups of patients and healthy subjects (p=0.042, p=0.036).
CONCLUSION
ATpA and PIKR may be used as biomarkers for early detection of VAP disease. However, further studies are required.
背景
呼吸机相关性肺炎（VAP）等感染性疾病是医院重症监护病房（ICU）的严重问题之一。迄今为止，尚无用于早期检测该疾病的合适临床和诊断标志物。在本研究中，评估VAP患者中PIKR和ATpA基因的表达，以用作识别和确诊该疾病的生物标志物。
方法
本研究使用60名个体的外周血样本进行，其中包括30名VAP患者和30名健康志愿者。首先采集外周血样本，然后提取RNA并转化为cDNA。最后，通过实时PCR对基因进行评估。
结果
在外周血样本中，患者组和健康组中PIKR表达阳性率分别为46.6%和30%。患者组和健康对照组中ATpA表达阳性率分别为40%和23.3%。比较PIKR和ATpA基因获得的ΔCT，两组患者与健康受试者之间存在统计学显著差异（p=0.042，p=0.036）。
结论
ATpA和PIKR可能用作早期检测VAP疾病的生物标志物。然而，还需要进一步研究。
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