Karrow Niel A, Lee Alison V, Quinton Margaret, McBride Brian W, Fisher-Heffernan Rebecca E
Department of Animal Biosciences, University of Guelph, Guelph N1G2W1, Canada.
Anim Nutr. 2017 Mar;3(1):39-45. doi: 10.1016/j.aninu.2016.10.001. Epub 2016 Oct 28.
Previous research has shown that adulthood disease can be attributed to stress events that occur during gestation. The objective of the present study was to determine whether maternal stress during late pregnancy, using a bacterial endotoxemia model, causes changes in hippocampal mRNA expression of candidate genes related to hypothalamic-pituitary-adrenal axis (HPAA) regulation in sheep. This study also sought to investigate whether maternal diets supplemented with fishmeal (FM) rich in omega-3 polyunsaturated fatty acids (PUFAs) offer protection to the fetus when subjected to maternal endotoxin stress. Using RT-qPCR, relative mRNA expression was assessed in both fetal lambs and 6-month-old lambs from dams supplemented with soybean meal (SM) or FM and challenged with either endotoxin or saline. From this it was found that fetal mineralocorticoid receptor () gene expression had a tendency to be altered during endotoxin challenge, however, in the 6-month-old offspring, and glucocorticoid receptor () gene expression were differently altered across treatment groups. These results suggest that gene regulation within the hippocampus is altered into adulthood by maternal endotoxin stress and that dietary supplementation affects outcome.
先前的研究表明，成年期疾病可归因于孕期发生的应激事件。本研究的目的是确定在妊娠晚期使用细菌内毒素血症模型时，母体应激是否会导致绵羊下丘脑 - 垂体 - 肾上腺轴（HPAA）调节相关候选基因的海马体mRNA表达发生变化。本研究还试图调查，当母体受到内毒素应激时，补充富含ω-3多不饱和脂肪酸（PUFA）的鱼粉（FM）的母体饮食是否能为胎儿提供保护。使用逆转录定量聚合酶链反应（RT-qPCR），对补充豆粕（SM）或FM并接受内毒素或生理盐水刺激的母羊所产的胎儿羔羊和6月龄羔羊的相对mRNA表达进行评估。由此发现，胎儿盐皮质激素受体（）基因表达在内毒素刺激期间有改变的趋势，然而，在6月龄后代中，不同处理组的和糖皮质激素受体（）基因表达有不同改变。这些结果表明，母体内毒素应激会使海马体内的基因调控在成年期发生改变，且饮食补充会影响结果。
