a Department of Physical Education and Sport Sciences , Aliabad Katoul Branch, Islamic Azad University , Aliabad Katoul , Iran.
b Department of Sport Physiology, College of Physical Education and Sport Sciences , University of Mazandaran , Babolsar , Iran.
Free Radic Res. 2018 Jul;52(7):775-782. doi: 10.1080/10715762.2018.1470328. Epub 2018 May 16.
The aims of the current study were to investigate the oxidant and antioxidant status of liver tissue challenged by doxorubicin and to examine the possible protective effects of aerobic exercise on doxorubicin-induced oxidative stress. Seventy-two rats were divided into three age groups (Young, Adult, and Elderly) with three treatment subgroups consisting of eight rats per age group: doxorubicin, aerobic exercise + doxorubicin, and aerobic exercise + saline. The experimental groups performed regular treadmill running for 3 weeks. Doxorubicin was administered by i.p. injection at a dosage of 20 mg kg while the aerobic exercise + saline group received saline of a comparable volume. Heat shock protein 70, malondialdehyde, glutathione peroxidase, and protein carbonyl were determined from the liver homogenates following the intervention period. Treatment with doxorubicin induced hepatotoxicity in all groups with lower values of oxidative stress in young compared with the older groups. The inclusion of aerobic exercise training significantly increased heat shock protein 70 and antioxidant enzyme levels (glutathione peroxidase) whereas it decreased oxidative stress biomarkers (malondialdehyde and protein carbonyl) for all age groups. These results suggest that aerobic exercise training may be a potential, non-drug strategy to modulate doxorubicin-induced hepatotoxicity through its positive impact on antioxidant levels and oxidative stress biomarkers.
本研究旨在探讨多柔比星对肝脏组织的氧化应激和抗氧化状态的影响，并观察有氧运动对多柔比星诱导的氧化应激的可能保护作用。72 只大鼠分为三组（青年、成年和老年），每组 8 只大鼠，分为三个治疗亚组：多柔比星组、有氧运动+多柔比星组和有氧运动+生理盐水组。实验组进行了为期 3 周的常规跑步机跑步。多柔比星通过腹腔注射给予 20mg/kg，而有氧运动+生理盐水组给予相同体积的生理盐水。干预期后，从肝匀浆中测定热休克蛋白 70、丙二醛、谷胱甘肽过氧化物酶和蛋白羰基。多柔比星治疗在所有组中均诱导了肝毒性，与老年组相比，年轻组的氧化应激值较低。有氧运动训练的纳入显著增加了热休克蛋白 70 和抗氧化酶水平（谷胱甘肽过氧化物酶），同时降低了所有年龄组的氧化应激生物标志物（丙二醛和蛋白羰基）。这些结果表明，有氧运动训练可能是一种潜在的非药物策略，通过对抗氧化水平和氧化应激生物标志物的积极影响来调节多柔比星诱导的肝毒性。
