Xiao-Fan Wang, Qian-Qian Qi, Li-Na Zhang, Lei Xu, Li-Yang Dong, Ya-Lin Li, Ya-Nan Pu, Chuan Wei, Sha Zhou, Ji-Feng Zhu, Xiao-Jun Chen, Chuan Su
Department of Pathogen Biology, Nanjing Medical University, Nanjing 211166, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2018 Apr 20;30(2):131-135. doi: 10.16250/j.32.1374.2018006.
To assess the influence of glycolytic pathway on the proportion and numbers of regulatory T cells during infection.
A -infected mouse model was established, and C57/BL6 male mice infected with were subjected to intraperitoneal injections of with the glycolytic inhibitor 2-Deoxy-D-glucose (2DG) or PBS for 6 times, and then the cells from spleen or mesenteric lymph nodes (LNs) were isolated and analyzed by flow cytometry (FCM) to detect the percentage of Glut1CD4 T cells and Treg cells.
The proportions of Glut1CD4 T cells in the spleen (43.58%±2.50% vs. 21.15%±0.96%; = 8.834, < 0.01) and mesenteric LNs (38.97%±1.97% vs. 28.40%±2.11%; = 3.662, < 0.05) were higher in the normal mice than those in the infected mice, and the percentages of Treg cells in the spleen (6.83%±0.21% vs. 13.30%±0.35%; = 15.65, < 0.01) and LNs (8.26%±0.15% vs. 14.37%±0.44%; = 13.14, < 0.01) were lower in the normal mice than those in the infected mice. In addition, the proportions of Treg cells in the spleen (15.50%±0.76% vs. 13.07%±0.15%; = 3.130, < 0.05) and LNs (17.00% ±0.41% vs. 13.83%±0.18%; = 6.947, < 0.01) were higher in the infected mice injected intraperitoneally with 2DG than those in the infected mice injected intraperitoneally with PBS.
Glycolytic pathway inhibits Treg differentiation in the spleen and mesenteric LNs of -infected mice.
评估糖酵解途径对感染期间调节性T细胞比例和数量的影响。
建立感染小鼠模型，将感染的C57/BL6雄性小鼠腹腔注射糖酵解抑制剂2-脱氧-D-葡萄糖（2DG）或磷酸盐缓冲液（PBS）6次，然后分离脾脏或肠系膜淋巴结（LN）中的细胞，通过流式细胞术（FCM）分析以检测Glut1CD4 T细胞和调节性T细胞的百分比。
正常小鼠脾脏中Glut1CD4 T细胞的比例（43.58%±2.50%对21.15%±0.96%；t = 8.834，P < 0.01）和肠系膜淋巴结中（38.97%±1.97%对28.40%±2.11%；t = 3.662，P < 0.05）高于感染小鼠，正常小鼠脾脏中调节性T细胞的百分比（6.83%±0.21%对13.30%±0.35%；t = 15.65，P < 0.01）和淋巴结中（8.26%±0.15%对14.37%±0.44%；t = 13.14，P < 0.01）低于感染小鼠。此外，腹腔注射2DG的感染小鼠脾脏中调节性T细胞的比例（15.50%±0.76%对13.07%±0.15%；t = 3.130，P < 0.05）和淋巴结中（17.00% ±0.41%对13.83%±0.18%；t = 6.947，P < 0.01）高于腹腔注射PBS的感染小鼠。
糖酵解途径抑制感染小鼠脾脏和肠系膜淋巴结中调节性T细胞的分化。
