Chen C H, Zhu G C, Pi L M, Wei M, She L, Tan H L, Liu G C, Liu Y, Zhang X
Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008, China.
Department of Otolaryngology Head and Neck Surgery, the Second Xiangya Hospital, Central South University.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2017 May 20;31(10):789-793. doi: 10.13201/j.issn.1001-1781.2017.10.013.
The aim of this study is to explore the effects of autophagy on the metastasis of the Squamous Cell Carcinoma of Head and Neck (SCCHN) via epithelial to mesenchymal transition (EMT) induced by TGF-β1. Establish the EMT model induced by TGF-β1 in the SCCHN in time/concentration, and the expression of autophagy related protein microtubule associated protein 1 light chain3 (LC3) detected by western blot; Autophagy inhibitor chloroquine (CQ), depressing autophagy, the expression of E-cadherin, cytokeratin, Vimentin and LC3 were examined by Western blot. Wound healing and Transwell invasion assay indicate the effects to metastasis for SCCHN. Autophagy was activated within TGF-β1 induced EMT model in the SCCHN in time/concentration dependently. After autophagy was suppressed, the expression of E-cadherin and cytokeratin increased while vimentin and the capacity of metastasis was reduced compared with control group. TGF-β1 induce EMT and Autophagy in the SCCHN. Autophagy could enhances metastasis in the SCCHN via EMT induced by TGF-β1.
本研究旨在探讨自噬通过转化生长因子-β1（TGF-β1）诱导的上皮-间质转化（EMT）对头颈鳞状细胞癌（SCCHN）转移的影响。及时/浓度依赖性地建立TGF-β1诱导的SCCHN中的EMT模型，并用蛋白质免疫印迹法检测自噬相关蛋白微管相关蛋白1轻链3（LC3）的表达；使用自噬抑制剂氯喹（CQ）抑制自噬，通过蛋白质免疫印迹法检测E-钙黏蛋白、细胞角蛋白、波形蛋白和LC3的表达。伤口愈合实验和Transwell侵袭实验表明其对SCCHN转移的影响。在TGF-β1诱导的SCCHN的EMT模型中，自噬呈时间/浓度依赖性激活。自噬被抑制后，与对照组相比，E-钙黏蛋白和细胞角蛋白的表达增加，波形蛋白表达及转移能力降低。TGF-β1在SCCHN中诱导EMT和自噬。自噬可通过TGF-β1诱导的EMT增强SCCHN的转移。
