Yamamoto A, Shouji T, Tamaki T, Fukui K, Iwao H, Abe Y
Department of Pharmacology, Kagawa Medical School, Japan.
J Hypertens Suppl. 1988 Dec;6(4):S317-9. doi: 10.1097/00004872-198812040-00098.
In order to examine the role of calcium in the secretory process of atrial natriuretic factor (ANF), we studied the effects of hypercalcaemia and ouabain on the plasma concentration of immunoreactive ANF, and the effect of calcium on immunoreactive ANF release from isolated rat atria. Anaesthetized dogs were treated with CaCl2 infusion, ouabain or phenylephrine injection. With CaCl2 infusion, serum calcium and plasma immunoreactive ANF respectively increased to three and four times their basal levels. Ouabain increased plasma immunoreactive ANF to two and a half times the initial level. Neither CaCl2 nor ouabain produced any effect on right atrial pressure and heart rate, but they both significantly increased arterial pressure. Phenylephrine caused a greater increase in arterial pressure than both CaCl2 and ouabain. However, there was no significant increase in plasma immunoreactive ANF. Moreover, calcium stimulated the release of immunoreactive ANF from isolated rat atria. These results suggest that the calcium may play a key role in the secretory process of ANF.
为了研究钙在心房利钠因子（ANF）分泌过程中的作用，我们研究了高钙血症和哇巴因对免疫反应性ANF血浆浓度的影响，以及钙对离体大鼠心房免疫反应性ANF释放的影响。对麻醉犬进行氯化钙输注、哇巴因或去氧肾上腺素注射处理。输注氯化钙后，血清钙和血浆免疫反应性ANF分别升至基础水平的三倍和四倍。哇巴因使血浆免疫反应性ANF升至初始水平的2.5倍。氯化钙和哇巴因对右心房压力和心率均无影响，但二者均显著升高动脉压。去氧肾上腺素引起的动脉压升高幅度大于氯化钙和哇巴因。然而，血浆免疫反应性ANF无显著升高。此外，钙刺激离体大鼠心房释放免疫反应性ANF。这些结果表明，钙可能在ANF的分泌过程中起关键作用。
