Selcuk University, Science Faculty, Department of Biology, Campus, 42250, Konya, Turkey.
Marmara University, Pharmacy Faculty, Department of Pharmaceutical Botany, Istanbul, Turkey.
Comput Biol Chem. 2018 Aug;75:111-119. doi: 10.1016/j.compbiolchem.2018.05.005. Epub 2018 May 6.
Salvia sclarea L. is traditionally used to manage common human ailments and is consumed as a food product. This study aimed to establish the phytochemical profile and antioxidant potential of ethyl acetate, methanol, and water extracts of Salvia sclarea. The inhibitory action of the extracts against α-amylase, α-glucosidase, acetylcholinesterase, butyrylcholinesterase, and tyrosinase was also investigated. Methanol extract showed the highest phenolic and flavonoid contents (81.78 mg GAE/g extract and 40.59 mg RE/g extract, respectively). Reversed phase high performance liquid chromatography with diode array detector analysis revealed that S. sclarea was rich in rosmarinic acid. The water extract exhibited the lowest inhibitory activity against α-amylase but the upmost activity against α-glucosidase (0.19 and 18.24 mmol ACAE/g extract, respectively). Experimental data showed that only the water extract (8.86 mg KAE/g extract) significantly inhibited tyrosinase. Docking studies showed that quercetin binds to tyrosinase by two hydrogen and a pi-pi bonds. Salvia sclarea showed interesting biological activity against key enzymes involved in the pathogenesis of common ailments.
薰衣草被传统上用于治疗常见的人类疾病，并作为食品消费。本研究旨在建立薰衣草的乙酸乙酯、甲醇和水提取物的植物化学成分谱和抗氧化潜力。还研究了提取物对α-淀粉酶、α-葡萄糖苷酶、乙酰胆碱酯酶、丁酰胆碱酯酶和酪氨酸酶的抑制作用。甲醇提取物表现出最高的酚类和类黄酮含量（分别为 81.78mg GAE/g 提取物和 40.59mg RE/g 提取物）。反相高效液相色谱-二极管阵列检测器分析表明，薰衣草富含迷迭香酸。水提取物对α-淀粉酶的抑制活性最低，但对α-葡萄糖苷酶的活性最高（分别为 0.19 和 18.24mmol ACAE/g 提取物）。实验数据表明，只有水提取物（8.86mg KAE/g 提取物）显著抑制了酪氨酸酶。对接研究表明，槲皮素通过两个氢键和一个 pi-pi 键与酪氨酸酶结合。薰衣草对参与常见疾病发病机制的关键酶表现出有趣的生物活性。
