三种盐生植物的生物和植物化学特性的体外和计算视角-来自自然界的创新植物药物的来源。

In vitro and in silico perspectives on biological and phytochemical profile of three halophyte species-A source of innovative phytopharmaceuticals from nature.

机构信息

Department of Biology, Faculty of Science, Selcuk University, Campus, Konya, Turkey.

Department of Health Sciences, Faculty of Science, University of Mauritius, Réduit, Mauritius.

出版信息

Phytomedicine. 2018 Jan 1;38:35-44. doi: 10.1016/j.phymed.2017.10.017. Epub 2017 Oct 31.

DOI:10.1016/j.phymed.2017.10.017

PMID:29425653

Abstract

BACKGROUND

Halophytes are considered as valuable sources of traditional drugs in different countries.

PURPOSE

The present study aimed to evaluate biological and chemical fingerprints of three halophytes (Arthrocnemum macrostachyum (Moric.) C, Koch, Halimione portulacoides (L.) Aellen and Salicornia europaea L.).

MATERIALS AND METHODS

The antioxidant and enzymatic inhibitory potential (acetylcholinesterase, butyrylcholinesterase, α-amylase, α-glucosidase, and tyrosinase) were assessed. The total phenolic, flavonoid contents, and the chemical profiles were appraised using the ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry. Molecular docking was conducted to provide additional insights of molecular interactions of the enzymes/phytochemicals.

RESULTS

Ethyl acetate extract was the most efficient extract, with A. macrostachyum being the most potent towards DPPH and ABTS radicals and phosphomolybdenum assay. Ethyl acetate extract of A. macrostachyum was also the best reducing agent (CUPRAC and FRAP assays). Methanol and ethyl acetate extract of A. macrostachyum, H. portulacoides, and S. europaea showed significant enzyme inhibition potential. Ethyl acetate extract of A. macrostachyum showed the highest total phenolic (29.54 ± 0.78 mgGAEs/g extract) while the ethyl acetate extract of S. europaea was more abundant in flavonoids (18.26 ± 0.11 mgREs/g extract). Phytochemical profiling allowed the identification of several components in the methanolic extracts (16 in A. macrostachyum, 14 in H. portulacoides, and 11 in S. europaea), with quinic acid, p-coumaric acid, and rhamnetin being most abundant. Docking studies revealed that the above compounds showed scores for the enzymes tested.

CONCLUSION

The three halophytes studies could be considered as potential sources of biologically-active compounds for novel phytopharmaceuticals development.

摘要

背景

盐生植物被认为是不同国家传统药物的宝贵来源。

目的

本研究旨在评估三种盐生植物（獐茅（Moric.）C. Koch、滨藜（L.）Aellen 和海蓬子 L.）的生物和化学指纹图谱。

材料与方法

评估了抗氧化和酶抑制潜力（乙酰胆碱酯酶、丁酰胆碱酯酶、α-淀粉酶、α-葡萄糖苷酶和酪氨酸酶）。使用超高效液相色谱-电喷雾串联质谱法评估总酚、类黄酮含量和化学图谱。进行分子对接以提供酶/植物化学物质分子相互作用的附加见解。

结果

乙酸乙酯提取物是最有效的提取物，獐茅对 DPPH 和 ABTS 自由基和磷钼酸盐测定的效果最强。獐茅的乙酸乙酯提取物也是最佳还原剂（CUPRAC 和 FRAP 测定）。獐茅、滨藜和海蓬子的甲醇和乙酸乙酯提取物均具有显著的酶抑制潜力。獐茅的乙酸乙酯提取物总酚含量最高（29.54±0.78 mgGAEs/g 提取物），而海蓬子的乙酸乙酯提取物则富含类黄酮（18.26±0.11 mgREs/g 提取物）。植物化学特征允许鉴定甲醇提取物中的几种成分（獐茅中有 16 种，滨藜中有 14 种，海蓬子中有 11 种），其中以奎宁酸、对香豆酸和鼠李糖苷最为丰富。对接研究表明，上述化合物对测试的酶表现出得分。