Jeng K C, Lan J L, Chou H F
Department of Internal Medicine, Taichung Veterans General Hospital, Taiwan, ROC.
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1988 Aug;21(3):151-7.
This study was designed to determine the suppressive effect of bovine brain gangliosides on primary and secondary anti-sheep red blood cell (SRBC) plaque-forming cells (PFC) response. Spleen cells from inbred C57BL/6J mice were used as sources of lymphocytes. The result showed that levels of gangliosides at 50 and 100 micrograms/ml suppressed primary anti-SRBC PFC responses about 30% and 60%, respectively, of normal control. In contrast, secondary anti-SRBC PFC were not suppressed by the same levels of gangliosides. Likewise, PFC response from mixing SRBC-primed T-cells with naive B-cells was not suppressed by gangliosides. In addition, induction of suppressor T-cells was reduced by 50 and 100 micrograms/ml of gangliosides, 57% and 63%, respectively, versus 86% of suppression of PFC response by the control suppressor T-cells. These results further suggest the role of gangliosides as immunomodulators.
本研究旨在确定牛脑神经节苷脂对初次和二次抗绵羊红细胞（SRBC）空斑形成细胞（PFC）反应的抑制作用。近交系C57BL/6J小鼠的脾细胞用作淋巴细胞来源。结果显示，50和100微克/毫升的神经节苷脂水平分别抑制初次抗SRBC PFC反应约正常对照的30%和60%。相比之下，相同水平的神经节苷脂并未抑制二次抗SRBC PFC反应。同样，将经SRBC致敏的T细胞与未致敏的B细胞混合后的PFC反应也未被神经节苷脂抑制。此外，50和100微克/毫升的神经节苷脂分别使抑制性T细胞的诱导减少57%和63%，而对照抑制性T细胞对PFC反应的抑制率为86%。这些结果进一步表明神经节苷脂作为免疫调节剂的作用。
