Reinertsen J L, Steinberg A D
J Immunol. 1977 Jul;119(1):217-22.
Supernatants from concanavalin A- (Con A) activated murine spleen cells have been shown to suppress the in vitro plaque-forming cell (PFC) response to sheep red blood cells (SRBC). The present study examined the effect of such Con A-activated spleen cell supernatants (herein termed CONS) on the in vivo immune response to SRBC in C57BL/6, BALB/c and CDF1 mice. CONS derived from BALB/c spleen cells suppressed direct PFC 4 and 8 days after immunization with 2 X 10(8) SRBC. CONS also suppressed indirect PFC 8 days after immunization, as well as serum hemagglutinins to SRBC. The PFC response of C57BL/6 (H-2b) mice was suppressed as much as that of BALB/c (H-2d) by CONS derived from BALB/c mice, indicating a lack of H-2 specificity of the CONS. In addition to suppression of the antibody response to SRBC, in vivo CONS administration resulted in reduction in spleen cell number. This reduction was not sufficient to explain the decreased PFC response. When the CONS was separated into less than 10,000 m.w. and greater than or equal to 10,000 m.w. fractions, the immunosuppressive activity was found in the less than 10,000 m.w. fraction. This observation suggests that intact interferon, SIRS, and MIF were not responsible for the results obtained.
刀豆蛋白A（Con A）激活的小鼠脾细胞培养上清液已被证明可抑制体外对绵羊红细胞（SRBC）的空斑形成细胞（PFC）反应。本研究检测了此类Con A激活的脾细胞培养上清液（以下简称CONS）对C57BL/6、BALB/c和CDF1小鼠体内对SRBC免疫反应的影响。来自BALB/c脾细胞的CONS在用2×10⁸个SRBC免疫后4天和8天抑制直接PFC。CONS在免疫后8天还抑制间接PFC以及对SRBC的血清血凝素。来自BALB/c小鼠的CONS对C57BL/6（H-2b）小鼠PFC反应的抑制程度与对BALB/c（H-2d）小鼠的抑制程度相同，表明CONS缺乏H-2特异性。除了抑制对SRBC的抗体反应外，体内给予CONS还导致脾细胞数量减少。这种减少不足以解释PFC反应的降低。当将CONS分离成分子量小于10000和大于或等于10000的组分时，发现免疫抑制活性存在于分子量小于10000的组分中。这一观察结果表明，完整的干扰素、SIRS和MIF并非导致所获结果的原因。
