Laboratory of Enzyme Chemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022, Vladivostok, 159, Prospect 100-let Vladivostoku, Russia(1).
Laboratory of Enzyme Chemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022, Vladivostok, 159, Prospect 100-let Vladivostoku, Russia(1); Far-Eastern Federal University, Vladivostok, 690022, 8, Sukhanova st., Russia.
Carbohydr Polym. 2018 Aug 1;193:189-195. doi: 10.1016/j.carbpol.2018.03.094. Epub 2018 Mar 30.
Enzymatic depolymerization of fucoidans attracts many researchers due to the opportunity of obtaining standardized fucoidan fragments. Fucoidanase catalyzes the cleavage of fucoidan from Fucus evanescens (FeF) to form low molecular weight products (LMP) and a polymeric fraction (HMP) with 50.8 kDa molecular weight and more than 50% yield. NMR spectroscopy shows that the HMP fraction has regular structure and consists of a repeating fragment [→3)-α-l-Fucp2,4OSO-(1 → 4)-α-l-Fucp2,4OSO-(1 → 4)-α-l-Fucp2OSO-(1→]. The anticancer effects of FeF fucoidan and its derivative (HMP) were studied in vitro on colon cancer cells HCT-116, HT-29, and DLD-1. The anticancer activity of the HMP fraction was found to be slightly lower than that of the FeF fucoidan. Research and practical applications of the enzyme include modification of native fucoidans for purposes of regular and easier characterized derivatives acquisition.
由于有机会获得标准化的褐藻糖胶片段，酶促褐藻糖胶解聚吸引了许多研究人员。褐藻糖胶酶催化从泡叶藻（FeF）中裂解褐藻糖胶，形成低分子量产物（LMP）和高分子量（HMP）聚合物片段，分子量为 50.8 kDa，产率超过 50%。NMR 光谱显示，HMP 片段具有规则的结构，由重复片段[→3）-α-l-岩藻糖基 2,4-O-磺酸基-（1→4）-α-l-岩藻糖基 2,4-O-磺酸基-（1→4）-α-l-岩藻糖基 2OSO-（1→]组成。在体外研究了泡叶藻褐藻糖胶及其衍生物（HMP）对结肠癌细胞 HCT-116、HT-29 和 DLD-1 的抗癌作用。发现 HMP 片段的抗癌活性略低于 FeF 褐藻糖胶。该酶的研究和实际应用包括对天然褐藻糖胶进行修饰，以获得具有规则和更容易表征的衍生物。
