Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Prospect 100 Let Vladivostok 159, Vladivostok, 690022, Russia.
Mar Drugs. 2013 Jul 11;11(7):2413-30. doi: 10.3390/md11072413.
Intracellular fucoidanase was isolated from the marine bacterium, Formosa algae strain KMM 3553. The first appearance of fucoidan enzymatic hydrolysis products in a cell-free extract was detected after 4 h of bacterial growth, and maximal fucoidanase activity was observed after 12 h of growth. The fucoidanase displayed maximal activity in a wide range of pH values, from 6.5 to 9.1. The presence of Mg2+, Ca2+ and Ba2+ cations strongly activated the enzyme; however, Cu2+ and Zn2+ cations had inhibitory effects on the enzymatic activity. The enzymatic activity of fucoidanase was considerably reduced after prolonged (about 60 min) incubation of the enzyme solution at 45 °C. The fucoidanase catalyzed the hydrolysis of fucoidans from Fucus evanescens and Fucus vesiculosus, but not from Saccharina cichorioides. The fucoidanase also did not hydrolyze carrageenan. Desulfated fucoidan from F. evanescens was hydrolysed very weakly in contrast to deacetylated fucoidan, which was hydrolysed more actively compared to the native fucoidan from F. evanescens. Analysis of the structure of the enzymatic products showed that the marine bacteria, F. algae, synthesized an α-l-fucanase with an endo-type action that is specific for 1→4-bonds in a polysaccharide molecule built up of alternating three- and four-linked α-l-fucopyranose residues sulfated mainly at position 2.
从海洋细菌 Formosa algae 菌株 KMM 3553 中分离到胞内岩藻聚糖酶。在细菌生长 4 小时后，首次在无细胞提取物中检测到岩藻聚糖酶解产物的出现，在生长 12 小时后观察到最大的岩藻聚糖酶活性。岩藻聚糖酶在 pH 值为 6.5 到 9.1 的较宽范围内显示出最大的活性。Mg2+、Ca2+和 Ba2+阳离子的存在强烈激活了该酶；然而，Cu2+和 Zn2+阳离子对酶活性有抑制作用。在 45°C 下长时间（约 60 分钟）孵育酶溶液后，岩藻聚糖酶的酶活性大大降低。岩藻聚糖酶催化褐藻胶、角叉菜胶和石花菜的褐藻聚糖水解，但不催化麒麟菜的褐藻聚糖水解。岩藻聚糖酶也不水解卡拉胶。与脱乙酰基褐藻胶相比，褐藻胶的脱硫产物的水解作用非常弱，而与褐藻胶相比，褐藻胶的脱乙酰基褐藻胶的水解作用更活跃。对酶解产物结构的分析表明，海洋细菌 Formosa algae 合成了一种具有内切作用的α-L-岩藻聚糖酶，该酶对由交替的三连接和四连接的α-L-岩藻糖吡喃糖残基组成的多糖分子中的 1→4-键具有特异性，主要在 2 位硫酸化。
