Liu Jianmin, Liu Beibei, Guo Yuanyuan, Chen Zhijun, Sun Wei, Gao Wuyue, Wu Hongliang, Wang Yan
Department of Urology, First Affiliated Hospital of Bengbu Medical College, Anhui Province, PR China.
Department of Urology, First Affiliated Hospital of Bengbu Medical College, Anhui Province, PR China.
Pathol Res Pract. 2018 Jun;214(6):806-813. doi: 10.1016/j.prp.2018.05.005. Epub 2018 May 9.
To explore the biological function and mechanism of miR-199a-3p in clear cell renal cell carcinoma (CCRCC).
We investigated the expression of miR-199a-3p in CCRCC through quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Over expression of miR-199a-3p was performed in CCRCC cell lines, and cell growth curve, colony formation capacity, cell invasion, wound healing and cell apoptosis assay were used for investigating the roles of miR-199a-3p in CCRCC.
The expression of miR-199a-3p in CCRCC tissues was significantly lower than that in para-carcinoma tissues. Functional assay showed that over expression of miR-199a-3p influenced cell growth, colony formation, cell invasion, cell migration and cell apoptosis in CCRCC cell lines.
Our work suggested that miR-199a-3p was related to cell growth, colony formation, cell invasion, cell migration and cell apoptosis, which might act as a tumor suppressor in CCRCC.
探讨miR-199a-3p在肾透明细胞癌（CCRCC）中的生物学功能及机制。
通过定量逆转录聚合酶链反应（qRT-PCR）检测miR-199a-3p在CCRCC中的表达。在CCRCC细胞系中过表达miR-199a-3p，并采用细胞生长曲线、集落形成能力、细胞侵袭、伤口愈合及细胞凋亡检测等方法研究miR-199a-3p在CCRCC中的作用。
miR-199a-3p在CCRCC组织中的表达明显低于癌旁组织。功能分析表明，miR-199a-3p过表达影响CCRCC细胞系的细胞生长、集落形成、细胞侵袭、细胞迁移及细胞凋亡。
我们的研究表明，miR-199a-3p与细胞生长、集落形成、细胞侵袭、细胞迁移及细胞凋亡相关，可能在CCRCC中发挥肿瘤抑制作用。
