Peoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St., Moscow, 117198, Russian Federation.
Peoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St., Moscow, 117198, Russian Federation; Shemyakin-Ovchinnikov Institute of bioorganic chemistry, Moscow, Russian Federation.
J Trace Elem Med Biol. 2018 Dec;50:629-633. doi: 10.1016/j.jtemb.2018.05.004.
Here we show the dependence of the unicellular biosensor S.ambigua lifespan on the water D/H isotopic composition. This dependence is bell-shaped with descents both in case of deficiency or excess of deuterium in water. The influence of the water D/H isotopic composition on the cell culture proliferative potential and colony forming efficiency in vitro was tested on the human dermal fibroblasts. We observed that the deuterium depleted water stimulates cell colony formation at the early passages. The dynamics of the cell doubling index in the deuterium depleted water-based growth medium showed higher proliferation potential compared to the water with normal isotopic composition. Using scratch assay, we have also studied the impact of the growth medium D/H isotopic composition on the cell motility of human cancer cell lines A549 and HT29. We have shown that the deuterium depleted water considerably suppressed cancer cell lines amoeboid movement in vitro.
在这里，我们展示了单细胞生物传感器 S.ambigua 的寿命对水的 D/H 同位素组成的依赖性。这种依赖性呈钟形，在水中氘缺乏或过量的情况下都会下降。我们测试了水的 D/H 同位素组成对体外人皮肤成纤维细胞培养增殖潜力和集落形成效率的影响。我们观察到，去氘水在早期传代时刺激细胞集落形成。与具有正常同位素组成的水相比，去氘水基生长培养基中的细胞倍增指数动力学显示出更高的增殖潜力。通过划痕实验，我们还研究了生长培养基 D/H 同位素组成对人癌细胞系 A549 和 HT29 细胞运动性的影响。我们已经表明，去氘水在体外显著抑制了癌细胞系的阿米巴样运动。
