, a CEBPE target involved in granulocytic differentiation.

Maturation of granulocytes is dependent on controlled gene expression by myeloid lineage restricted transcription factors. CEBPE is one of the essential transcription factors required for granulocytic differentiation. Identification of downstream targets of CEBPE is vital to understand better its role in terminal granulopoiesis. In this study, we have identified as a novel target of CEBPE. We show that CEBPE binds to regulatory elements upstream of the murine locus, and expression of CARD10 is significantly reduced in knock-out mice. Silencing in a human cell line and in murine primary cells impaired granulopoiesis, affecting expression of genes involved in myeloid cell development and function. Taken together, our data demonstrate for the first time that is expressed in granulocytes and is a direct target of CEBPE with functions extending to myeloid differentiation.