Lee Daewon, Lee Amos Chungwon, Han Sangkwon, Bae Hyung Jong, Song Seo Woo, Jeong Yunjin, Oh Dong Yoon, Cho Seongkyu, Kim Junhoi, Park Wook, Kwon Sunghoon
Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 08826, South Korea.
Department of Electrical and Computer Engineering, Seoul National University, Seoul 08826, South Korea.
Biomicrofluidics. 2018 May 4;12(3):031102. doi: 10.1063/1.5030597. eCollection 2018 May.
Understanding tissue engineering using a bottom-up approach has been hindered by technical limitations because no platform can demonstrate the controlled formation of a heterogeneous population of cells in microscale. Here, we demonstrate hierarchical shape-by-shape assembly of virus-laden particles into larger ones to transfect two different genes on the seeded cells. We show that smaller daughter particles with different sizes and shapes can be assembled into the matching indentations of larger parent particles with different sizes and shapes. Then, we transfected a population of cells with two different gene-transfecting viruses, each of which was laden on the parent or daughter particles.
由于技术限制，采用自下而上的方法理解组织工程一直受到阻碍，因为没有平台能够在微观尺度上展示异质细胞群体的可控形成。在这里，我们展示了将载有病毒的颗粒逐个层次地组装成更大的颗粒，以便在接种的细胞上转染两种不同的基因。我们表明，具有不同大小和形状的较小子颗粒可以组装到具有不同大小和形状的较大母颗粒的匹配凹痕中。然后，我们用两种不同的基因转染病毒转染一群细胞，每种病毒都负载在母颗粒或子颗粒上。
