Chae Y, Yang M, Kim J
Private Practice, Baltimore, MD, USA.
Regenerative Health Research Laboratory, Philadelphia, PA, USA.
Int Endod J. 2018 Dec;51(12):1389-1397. doi: 10.1111/iej.12951. Epub 2018 Jun 9.
To investigate the release of growth factors into the root canal space after various final irrigants during regenerative endodontic procedures. The residual cytotoxic effect of final irrigants on stem cells from the apical papilla (SCAP) was also examined.
To measure the release of TGF-β1, root segments (8 mm long) were irrigated with 1.5% NaOCl followed by 20 mL of final irrigants; Saline, 17% EDTA, 10% citric acid, 10% or 37% phosphoric acid. Specimens were then immersed into culture medium for 24 h and the supernatants were collected to measure TGF-β1 by ELISA. For the cytotoxicity of residual final irrigants, dentine chips (5 × 5 × 1 mm) treated with irrigants as above were placed in the upper chamber of transwell system. Stem cells from the apical papilla were incubated indirectly in the lower chamber for 24 h and MTS assay was performed after 24 h. The surfaces of irrigated root canals were examined for smear layer with a scanning electron microscope (SEM). Log transformation was performed for ELISA data to compare different groups (one-way ANOVA, α = 0.05).
Ten percent citric acid released the greatest amount of TGF-β1 amongst all groups, which was significantly different to 17% EDTA (P < 0.01). All dentine chips irrigated with the irrigants showed no significant difference of cytotoxicity on SCAP compared to nonirrigated dentine (P > 0.05). SEM revealed completely open dentinal tubules in 10% citric acid, whereas 17% EDTA was associated with partially open dentinal tubules.
Ten percent citric acid was effective as a final irrigant for releasing TGF-β1 with good biocompatibility in regenerative endodontics.
研究在再生性牙髓治疗过程中，不同的终末冲洗剂作用后生长因子在根管内的释放情况。同时检测终末冲洗剂对根尖乳头干细胞（SCAP）的残余细胞毒性作用。
为测量转化生长因子-β1（TGF-β1）的释放，用1.5%次氯酸钠冲洗8毫米长的牙根片段，随后用20毫升终末冲洗剂冲洗；生理盐水、17%乙二胺四乙酸（EDTA）、10%柠檬酸、10%或37%磷酸。然后将样本浸入培养基中24小时，收集上清液并用酶联免疫吸附测定（ELISA）法检测TGF-β1。对于残余终末冲洗剂的细胞毒性，将经上述冲洗剂处理的牙本质碎屑（5×5×1毫米）置于Transwell系统的上室。根尖乳头干细胞在下室间接培养24小时，24小时后进行噻唑蓝比色法（MTS）检测。用扫描电子显微镜（SEM）检查冲洗后的根管表面的玷污层。对ELISA数据进行对数转换以比较不同组（单因素方差分析，α = 0.05）。
在所有组中，10%柠檬酸释放的TGF-β1量最大，与17% EDTA有显著差异（P < 0.01）。与未冲洗的牙本质相比，所有经冲洗剂冲洗的牙本质碎屑对SCAP的细胞毒性均无显著差异（P > 0.05）。SEM显示10%柠檬酸处理后的牙本质小管完全开放，而17% EDTA处理后的牙本质小管部分开放。
10%柠檬酸作为终末冲洗剂在再生性牙髓治疗中能有效释放TGF-β1，且具有良好的生物相容性。
