Gugliani Abhilasha, Taneja Sonali, Shetty Devi Charan, Bhalla Vidhi Kiran
Consultant Endodontist, ITS Centre for Dental Studies and Research, Uttar Pradesh, India.
Department of Conservative Dentistry and Endodontics, ITS Centre for Dental Studies and Research, Uttar Pradesh, India.
Eur Endod J. 2025 Jan;10(1):1-10. doi: 10.14744/eej.2024.84856.
The aim of this study was to evaluate and compare the effect of various disinfection protocols on bacterial biofilm and subsequent release of growth factors from radicular dentin.
One hundred and ninety two extracted single rooted premolars were obtained and contaminated with E. faecalis biofilm for 21 days. The samples were then divided into three main groups - Group I: Irrigation (I) only, Group II: Calcium hydroxide (CH) placement followed by final irrigation and Group III: Triple Antibiotic paste (TAP) placement followed by final irrigation. Each group was further then divided into four sub-groups according to the final irrigating solution used - Sub group A: Saline, Sub group B: 17% EDTA, Sub group C: 1% phytic acid and Sub group D: 0.2%. chitosan nanoparticles. After treatment, the samples were subjected to colony-forming unit (CFU) analysis to determine bacterial reduction and the release of TGF-β1 and VEGF from the root canals, which was quantified using Enzyme-Linked Immunosorbent Assay (ELISA). The data were analyzed using statistical tests.
The maximum reduction in E. faecalis biofilm was observed in Group III (TAP), followed by Group II (CH), and finally Group I (irrigation only). Among the subgroups, the maximum reduction in bacterial biofilm was seen with chitosan nanoparticles, followed by phytic acid, EDTA, and saline. After 24 hours, the highest release of both TGF-β1 and VEGF was observed in the chitosan nanoparticles subgroup, followed by phytic acid, EDTA, and saline. Similar results were seen in the CH and TAP groups.
The study concluded that newer irrigating solutions, particularly 0.2% chitosan nanoparticles, showed superior antibacterial activity and better smear layer removal, leading to greater growth factor release from the radicular dentin. The study also highlighted that TAP placement resulted in maximum bacterial reduction, regardless of the final irrigant used. Furthermore, the release of TGF-β1 was significantly higher than VEGF in all groups. (EEJ-2024-03-045).
本研究旨在评估和比较不同消毒方案对细菌生物膜以及随后从根管牙本质中释放生长因子的影响。
获取192颗拔除的单根前磨牙，用粪肠球菌生物膜污染21天。然后将样本分为三个主要组——第一组：仅冲洗（I），第二组：放置氢氧化钙（CH）后进行最终冲洗，第三组：放置三联抗生素糊剂（TAP）后进行最终冲洗。然后根据最终使用的冲洗液将每组进一步分为四个亚组——A亚组：生理盐水，B亚组：17%乙二胺四乙酸（EDTA），C亚组：1%植酸，D亚组：0.2%壳聚糖纳米颗粒。处理后，对样本进行菌落形成单位（CFU）分析以确定细菌减少情况以及根管中转化生长因子-β1（TGF-β1）和血管内皮生长因子（VEGF）的释放，使用酶联免疫吸附测定（ELISA）对其进行定量。使用统计检验对数据进行分析。
在第三组（TAP）中观察到粪肠球菌生物膜减少最多，其次是第二组（CH），最后是第一组（仅冲洗）。在亚组中，壳聚糖纳米颗粒使细菌生物膜减少最多，其次是植酸、EDTA和生理盐水。24小时后，壳聚糖纳米颗粒亚组中TGF-β1和VEGF的释放最高，其次是植酸、EDTA和生理盐水。在CH组和TAP组中也观察到类似结果。
该研究得出结论，新型冲洗液，特别是0.2%壳聚糖纳米颗粒，显示出卓越的抗菌活性和更好的玷污层去除效果，导致从根管牙本质中释放更多生长因子。该研究还强调，无论最终使用何种冲洗剂，放置TAP都能使细菌减少最多。此外，在所有组中TGF-β1的释放均显著高于VEGF。（EEJ - 2024 - 03 - 045）
