Vetsuisse Faculty, Institute of Genetics, University of Bern, 3001, Bern, Switzerland.
ProSpecieRara, Basel, Switzerland.
Anim Genet. 2018 Aug;49(4):334-336. doi: 10.1111/age.12674. Epub 2018 May 18.
The crest in chicken consists of elongated and upraised feathers, as seen in various breeds such as the Silkie chicken. Recently, the still unknown causative mutation for the crest phenotype was assigned to chromosome 33 and an ectopic expression of HOXC8 was shown. The aim this study was to evaluate whether the crest phenotype in a local Swiss chicken breed, the Appenzeller Spitzhaubenhuhn, is associated with HOXC8. Three previously reported crest-associated flanking markers at the HOXC8 locus were genotyped in cohorts of this breed and two other local breeds without the crest phenotype. For the Appenzeller Spitzhaubenhuhn chicken showing the crest phenotype, no clear association of the reported markers could be revealed. Furthermore, the two exons of HOXC8 were sequenced in crested chicken of the Appenzeller Spitzhaubenhuhn and Silkie breeds and revealed no evidence of polymorphisms within the coding region of HOXC8. Therefore, the molecular genetic etiology for the crest phenotype in the investigated breeds remains unclear.
鸡的鸡冠由拉长和竖起的羽毛组成，如丝毛鸡等各种品种可见。最近，鸡冠表型的致病突变仍不明，被分配到第 33 号染色体，并显示出 HOXC8 的异位表达。本研究的目的是评估瑞士本地品种阿彭策尔卷毛鸡的鸡冠表型是否与 HOXC8 有关。在这个品种和另外两个没有鸡冠表型的本地品种的群体中，对 HOXC8 基因座上三个先前报道的与鸡冠相关的侧翼标记进行了基因分型。对于表现出鸡冠表型的阿彭策尔卷毛鸡，未发现报道的标记有明显的关联。此外，对阿彭策尔卷毛鸡和丝毛鸡的鸡冠鸡的 HOXC8 的两个外显子进行测序，未发现 HOXC8 编码区的多态性证据。因此，所研究品种的鸡冠表型的分子遗传病因仍不清楚。
