Department of Fundamental & Applied Science, Universiti Teknologi PETRONAS, 32610 Seri Iskandar, Perak Darul Ridzuan, Malaysia.; Centre of Innovative Nanostructure & Nanodevices (COINN), Universiti Teknologi PETRONAS, 32610 Seri Iskandar, Perak Darul Ridzuan, Malaysia.
School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600 Arau, Perlis, Malaysia; Institute of Nano Electronic Engineering, University Malaysia Perlis, 01000 Kangar, Perlis, Malaysia.
Int J Biol Macromol. 2018 Sep;116:765-773. doi: 10.1016/j.ijbiomac.2018.05.084. Epub 2018 May 15.
There are different clotting factors present in blood, carries the clotting cascade and excessive bleeding may cause a deficiency in the clotting Diagnosis of this deficiency in clotting drastically reduces the potential fatality. For enabling a sensor to detect the clotting factors, suitable probes such as antibody and aptamer have been used to capture these targets on the sensing surface. Two major clotting factors were widely studied for the diagnosis of clotting deficiency, which includes factor IX and thrombin. In addition, factor IX is considered as the substitute for heparin and the prothrombotic associated with the increased thrombin generation are taking into account their prevalence. The biosensors, surface plasmon resonance, evanescent-field-coupled waveguide-mode sensor, metal-enhanced PicoGreen fluorescence and electrochemical aptasensor were well-documented and improvements have been made for high-performance sensing. We overviewed detecting factor IX and thrombin using these biosensors, for the potential application in medical diagnosis.
血液中存在不同的凝血因子，它们参与凝血级联反应。过度出血可能导致凝血因子缺乏。这种凝血因子缺乏的诊断可以大大降低潜在的死亡率。为了使传感器能够检测凝血因子，已经使用了合适的探针，如抗体和适体，以在传感表面捕获这些靶标。因子 IX 和凝血酶这两种主要的凝血因子被广泛研究用于诊断凝血因子缺乏症。此外，因子 IX 被认为是肝素的替代品，与增加的凝血酶生成相关的促血栓形成因子也被考虑在内，因为它们很常见。基于表面等离子体共振、倏逝场耦合波导模式传感器、金属增强 PicoGreen 荧光和电化学生物传感器的生物传感器已经有了很好的记录，并且已经进行了改进以实现高性能传感。我们综述了使用这些生物传感器检测因子 IX 和凝血酶的方法，以期在医学诊断中得到应用。
