Soleimanifar Fatemeh, Mortazavi Yousef, Nadri Samad, Islami Maryam, Vakilian Saeid
Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran.
Department of Medical Biotechnology and Nanotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran; Cancer Gene Therapy Research Center, Zanjan University of Medical Sciences, Zanjan, Iran.
Biologicals. 2018 Jul;54:39-43. doi: 10.1016/j.biologicals.2018.04.005.
Coculture systems are widely used in tissue engineering to mimic cell-cell interactions between different populations. This study aimed to find an improved and convenient system for the corneal epithelial differentiation of conjunctiva derived mesenchymal stem cells (CJMSCs). Thus, the cells were used to reconstruct corneal epithelial cells. Obtained by flow cytometry data, 51.9% of isolated CJMSCs were immune reactive for SSEA4+ antibody which are more potent to differentiate into corneal epithelial cells. A differential medium in a single culture plate was applied and compared to a Coculture with a SHEM medium and a Coculture with a commercial medium. It was found that CJMSCs can be induced to corneal epithelial cells through in vitro co-culturing in a SHEM medium; this was confirmed with immunostaining results. Moreover, relative gene expression results showed that a Coculture system with a SHEM medium can provide a more favorable microenvironment for cells to differentiate into epithelial cells than a single culture or a Coculture with a CnT-Prime commercial medium. Finally, as platforms for cell differentiation, CJMSCs can differentiate into epithelial lineages. This was proven using immunofluorescence staining.
共培养系统在组织工程中被广泛应用，以模拟不同细胞群体之间的细胞-细胞相互作用。本研究旨在找到一种改良且便捷的系统，用于结膜来源的间充质干细胞（CJMSCs）的角膜上皮分化。因此，这些细胞被用于重建角膜上皮细胞。通过流式细胞术获得的数据显示，51.9%的分离CJMSCs对SSEA4+抗体呈免疫反应性，这些细胞更有潜力分化为角膜上皮细胞。在单个培养板中应用差异培养基，并与使用SHEM培养基的共培养以及使用商业培养基的共培养进行比较。结果发现，通过在SHEM培养基中体外共培养，CJMSCs可被诱导分化为角膜上皮细胞；免疫染色结果证实了这一点。此外，相对基因表达结果表明，与单一培养或使用CnT-Prime商业培养基的共培养相比，使用SHEM培养基的共培养系统可为细胞分化为上皮细胞提供更有利的微环境。最后，作为细胞分化平台，CJMSCs可分化为上皮谱系。这通过免疫荧光染色得以证明。
