Department of Biological Science and Technology, School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, China.
Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Int J Clin Oncol. 2018 Oct;23(5):867-876. doi: 10.1007/s10147-018-1295-2. Epub 2018 May 18.
Currently, the MDM2 promoter rs937283 A > G variant that is able to alter MDM2 gene expression has been widely studied to explore the association of MDM2 with cancer risk. In this report, we investigate the association of MDM2 rs937283 A > G variant with risk of lung cancer (LC) and gastric cancer (GC) in a Chinese population of Hubei province, which was followed by a meta-analysis.
The genotyping of rs937283 was performed by polymerase chain reaction-restriction fragment length polymorphism and confirmed by sequencing.
The results of the present study showed that rs937283 was significantly associated with the risk of LC, and the factors of age, gender, smoking status and drinking status would affect such association. However, rs937283 was only associated with the risk of GC in male, smoking and drinking subgroups. The following meta-analysis demonstrated that rs937283 was associated with the overall cancer risk particularly in Chinese population, which reinforced our present finding. Moreover, the meta-analysis according to cancer types revealed that rs937283 was associated with retinoblastoma risk, but not squamous cell carcinoma risk.
Collectively, the MDM2 rs937283 A > G variant may be a valuable risk factor or diagnostic biomarker for Chinese cancer patients.
目前，能够改变 MDM2 基因表达的 MDM2 启动子 rs937283A>G 变体已被广泛研究，以探讨 MDM2 与癌症风险的关系。在本报告中，我们调查了 MDM2 rs937283A>G 变体与湖北汉族人群肺癌（LC）和胃癌（GC）风险的关联，并进行了荟萃分析。
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法进行 rs937283 基因分型，并通过测序进行验证。
本研究结果表明，rs937283 与 LC 风险显著相关，年龄、性别、吸烟状况和饮酒状况等因素会影响这种关联。然而，rs937283 仅与男性、吸烟和饮酒亚组的 GC 风险相关。随后的荟萃分析表明，rs937283 与总体癌症风险相关，特别是在中国人群中，这进一步证实了我们的发现。此外，根据癌症类型进行的荟萃分析表明，rs937283 与视网膜母细胞瘤风险相关，但与鳞状细胞癌风险无关。
综上所述，MDM2 rs937283A>G 变体可能是中国癌症患者有价值的风险因素或诊断生物标志物。
