一种从变形链球菌OMZ 176中高产纯化葡糖基转移酶复合物的简单方法。
A simple purification method for a glucosyltransferase complex from Streptococcus mutans OMZ 176 with a high yield.
作者信息
Furutani M, Iwaki M, Yagi T, Iida M, Horiike K, Nozaki M
机构信息
Department of Oral and Maxillofacial Surgery, Shiga University of Medical Science, Japan.
出版信息
Int J Biochem. 1988;20(11):1327-32. doi: 10.1016/0020-711x(88)90238-8.
PMID:2977765
Abstract
- Glucosyltransferase was purified from Streptococcus mutans OMZ 176 by ammonium sulfate fractionation and gel filtration. 2. The overall purification was about 15,000-fold with a yield of 53%. 3. The purified enzyme appeared to be a multienzyme complex consisting of three different components with molecular weights of 158,000, 153,000 and 149,000, respectively.
摘要
- 通过硫酸铵分级分离和凝胶过滤从变形链球菌OMZ 176中纯化葡糖基转移酶。2. 总体纯化倍数约为15000倍，产率为53%。3. 纯化后的酶似乎是一种多酶复合物，由分子量分别为158000、153000和149000的三种不同组分组成。
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