Fukui K, Fukui Y, Moriyama T
J Bacteriol. 1974 Jun;118(3):796-804. doi: 10.1128/jb.118.3.796-804.1974.
Invertase (beta-d-fructofuranoside fructohydrolase, EC 3.2.1.26) and dextransucrase (alpha-1, 6-glucan: d-fructose 2-glucosyltransferase, EC 2.4.1.5) were purified from the culture fluids of Streptococcus mutans by chromatography on Sepharose 6B and diethylaminoethyl-cellulose followed by treatment with hydroxyapatite. Each of the enzyme preparations gave a single band when analyzed by either polyacrylamide gel electrophoresis or immunodiffusion. The antigenic determinant of invertase was different from that of dextransucrase on immunodiffusion. The pH optima were 5.25 for invertase and 5.75 for dextransucrase, and the K(m) values were 20 mM for invertase and 2.0 mM for dextransucrase. The molecular weights determined by sodium dodecyl sulfate gel electrophoresis were 160,000 for invertase and 170,000 for dextransucrase. The data obtained suggest that the dextransucrase had dextran-synthesizing activity and invertase-like activity.
通过在琼脂糖6B和二乙氨基乙基纤维素上进行色谱分离，随后用羟基磷灰石处理，从变形链球菌的培养液中纯化出了转化酶（β-D-呋喃果糖苷果糖水解酶，EC 3.2.1.26）和葡聚糖蔗糖酶（α-1,6-葡聚糖：D-果糖2-葡糖基转移酶，EC 2.4.1.5）。通过聚丙烯酰胺凝胶电泳或免疫扩散分析时，每种酶制剂都呈现出单一的条带。在免疫扩散中，转化酶的抗原决定簇与葡聚糖蔗糖酶的不同。转化酶的最适pH值为5.25，葡聚糖蔗糖酶的最适pH值为5.75，转化酶的K(m)值为20 mM，葡聚糖蔗糖酶的K(m)值为2.0 mM。通过十二烷基硫酸钠凝胶电泳测定的分子量，转化酶为160,000，葡聚糖蔗糖酶为170,000。所获得的数据表明，葡聚糖蔗糖酶具有葡聚糖合成活性和类似转化酶的活性。
