Yang Yalong, Zahr Kher, Harding Michael W, Strelkov Stephen E, Zuzak Krista, Feindel David, Feng Jie
Alberta Plant Health Lab, Crop Diversification Centre North, Alberta Agriculture and Forestry, Edmonton, Alberta T5Y 6H3, Canada.
Crop Diversification Centre South, Alberta Agriculture and Forestry, Brooks, Alberta T1R 1E6, Canada.
J Microbiol Methods. 2018 Jun;149:120-122. doi: 10.1016/j.mimet.2018.05.011. Epub 2018 May 16.
A commonly used protocol for DNA extraction from Plasmodiophora brassicae was modified by adding an alkaline treatment step to increase the purity of resting spores. The quality of DNA extracted by the modified protocol was improved due to the removal of DNA contamination from host plant cells and other microorganisms.
一种常用的从芸苔根肿菌中提取DNA的方法通过增加一个碱性处理步骤进行了改进，以提高休眠孢子的纯度。由于去除了来自宿主植物细胞和其他微生物的DNA污染，经改进的方法提取的DNA质量得到了提高。
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