Dpto. Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040, Madrid, Spain.
Dpto. Sanidad Animal. Facultad de Veterinaria, Universidad Complutense de Madrid (UCM), 28040, Madrid, Spain.
Vet Microbiol. 2018 Jun;219:123-127. doi: 10.1016/j.vetmic.2018.04.027. Epub 2018 Apr 17.
PRRSV can replicate for months in lymphoid organs leading to persistent host infections. Porcine bone marrow comprises two major monocyte subsets, one of which expresses CD163 and CD169, two receptors involved in the entry of PRRSV in macrophages. In this study, we investigate the permissiveness of these subsets to PRRSV infection. PRRSV replicates efficiently in BM CD163 monocytes reaching titers similar to those obtained in alveolar macrophages, but with a delayed kinetics. Infection of BM CD163 monocytes was variable and yielded lower titers. This may be related with the capacity of BM CD163 monocytes to differentiate into CD163 CD169 cells after culture in presence of M-CSF. Both subsets secreted IL-8 in response to virus but CD163 cells tended to produce higher amounts. The infection of BM monocytes by PRRSV may contribute to persistence of the virus in this compartment and to hematological disorders found in infected animals such as the reduction in the number of peripheral blood monocytes.
PRRSV 可在淋巴器官中复制数月，导致持续性宿主感染。猪骨髓包含两个主要的单核细胞亚群，其中一个表达 CD163 和 CD169，这两个受体参与了 PRRSV 进入巨噬细胞。在本研究中，我们研究了这些亚群对 PRRSV 感染的易感性。PRRSV 在 BM CD163 单核细胞中高效复制，达到与肺泡巨噬细胞相似的滴度，但动力学延迟。BM CD163 单核细胞的感染具有变异性，产生的滴度较低。这可能与 BM CD163 单核细胞在 M-CSF 存在的情况下培养后分化为 CD163 CD169 细胞的能力有关。两个亚群都能分泌 IL-8 来响应病毒，但 CD163 细胞倾向于产生更高的量。PRRSV 感染 BM 单核细胞可能有助于病毒在该部位的持续存在，并导致感染动物出现血液学紊乱，例如外周血单核细胞数量减少。
