Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo, A.C. Carretera a la Victoria km 0.6 C.P. 83304, Hermosillo, Sonora, Mexico.
Departamento de Ciencias Químico Biológicas, Universidad de Sonora, 83000, Hermosillo, Sonora, Mexico.
Vet Microbiol. 2018 Sep;223:27-33. doi: 10.1016/j.vetmic.2018.07.012. Epub 2018 Jul 19.
Porcine reproductive and respiratory syndrome virus (PRRSV) is the most important disease affecting the swine industry worldwide. Although monocytes and macrophages, especially tissue-resident and alveolar macrophages, are the primary target of PRRSV, monocyte- and bone marrow-derived dendritic cells (DCs) are also susceptible to PRRSV infection. It has been shown that lung DCs cannot be infected with PRRSV, but the response and susceptibility of bona fide conventional DC subtypes (cDCs; cDC1 and cDC2) is unknown. In this work, evaluation of the response of tracheal cDC1 and cDC2 subsets to PRRSV revealed differential cytokine expression, whereby cDC1 subsets expressed higher levels of IFN-α and cDC2 subsets more IL-10. Toll-like receptors (TLRs) were also affected: cDC2 cells induced greater upregulation of TLR2 and TLR4, and CD163 cells showed TLR3 upregulation. However, we could not demonstrate under our experimental conditions that cDC1 and cCD2 subsets are susceptible to PRRSV infection. Our findings show the effects of PRRSV on cDC1 and cDC2 subsets and that these cells were not infected by PRRSV.
猪繁殖与呼吸综合征病毒（PRRSV）是全球范围内影响养猪业最重要的疾病。虽然单核细胞和巨噬细胞，特别是组织驻留的和肺泡巨噬细胞，是 PRRSV 的主要靶标，但单核细胞和骨髓来源的树突状细胞（DCs）也容易感染 PRRSV。已经表明，肺 DCs 不能被 PRRSV 感染，但真正的常规 DC 亚型（cDCs；cDC1 和 cDC2）的反应和易感性尚不清楚。在这项工作中，评估了 PRRSV 对气管 cDC1 和 cDC2 亚群的反应，发现细胞因子表达存在差异，其中 cDC1 亚群表达更高水平的 IFN-α，而 cDC2 亚群表达更多的 IL-10。Toll 样受体（TLRs）也受到影响：cDC2 细胞诱导更高水平的 TLR2 和 TLR4 上调，而 CD163 细胞显示 TLR3 上调。然而，在我们的实验条件下，我们无法证明 cDC1 和 cCD2 亚群是否容易感染 PRRSV。我们的研究结果表明 PRRSV 对 cDC1 和 cDC2 亚群的影响，以及这些细胞不受 PRRSV 感染。
