Assisted Reproductive Technology Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, SAR China.
Assisted Reproductive Technology Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, SAR China; Li Ka Shing Institute of Health Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, SAR China; School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, SAR China.
Fertil Steril. 2018 May;109(5):832-839. doi: 10.1016/j.fertnstert.2018.01.022.
To compare the prevalence of chronic endometritis (CE) when different diagnostic methods are used.
Prospective observational study.
University-affiliated hospital.
PATIENT(S): Four groups of women were studied, including women with proven fertility (Fertile; n = 40), unexplained recurrent miscarriage (RM; n = 93), recurrent implantation failure (RIF; n = 39), and infertile subjects undergoing endometrial scratch in a natural cycle preceding frozen-thawed embryo transfer (Infertility; n = 48).
INTERVENTION(S): Endometrial biopsy was performed precisely 7 days after LH surge (LH+7). Plasma cells were identified by means of traditional hematoxylin and eosin (HE) staining and by means of immunohistochemistry (IHC) for Syndecan-1 (CD138).
MAIN OUTCOME MEASURE(S): Prevalence of CE.
RESULT(S): The use of CD138 epitope was more sensitive than HE staining in identifying plasma cells. The use of plasma cell count per unit area had the lowest observer variability compared with cell count per ten randomly chosen high-power fields and cell count per section. Using this method, the prevalence of CE in women with RM, RIF, and Infertility were 10.8%, 7.7%, and 10.4%, respectively, not significantly higher than that of Fertile subjects (5.0%).
CONCLUSION(S): Using what may be a new method of plasma cell assessment, it appears that the prevalence rates of CE reported in many earlier studies may have been overestimated.
ChiCTR-IOC-16007882.
比较不同诊断方法检测慢性子宫内膜炎（CE）的患病率。
前瞻性观察性研究。
大学附属医院。
研究纳入了四组患者，包括有明确生育能力的女性（生育组；n=40）、不明原因复发性流产（RM；n=93）、复发性着床失败（RIF；n=39）和在自然周期进行子宫内膜搔刮以准备冻融胚胎移植的不孕患者（不孕组；n=48）。
在 LH 峰后 7 天（LH+7）精确进行子宫内膜活检。通过传统苏木精和伊红（HE）染色和免疫组织化学（IHC）检测 Syndecan-1（CD138）来鉴定浆细胞。
CE 的患病率。
与 HE 染色相比，CD138 表位的使用在鉴定浆细胞方面更敏感。与每 10 个随机选择的高倍视野的细胞计数和每部分的细胞计数相比，单位面积浆细胞计数的观察者变异性最低。使用这种方法，RM、RIF 和不孕患者中 CE 的患病率分别为 10.8%、7.7%和 10.4%，与生育组（5.0%）相比无显著差异。
使用可能是一种新的浆细胞评估方法，似乎许多早期研究报告的 CE 患病率可能被高估了。
ChiCTR-IOC-16007882。
