Institute of Medical Microbiology, Virology, and Hygiene, Rostock University Medical Centre, Schillingallee 70, 18057 Rostock, Germany.
Department Cell Biology, Rostock University Medical Centre, Schillingallee 69, 18057 Rostock, Germany.
Int J Biochem Cell Biol. 2018 Jul;100:49-53. doi: 10.1016/j.biocel.2018.05.008. Epub 2018 May 17.
Extracellular vesicles are produced by a number of different cell types, among them mesenchymal stromal/stem cells (MSC) of different sources. It has been shown that extracellular vesicles of MSC exert similar therapeutic effects as the cells themselves. Here, we isolated and characterized extracellular vesicles produced by adipose-derived MSC (adMSC) in vitro upon stimulation with the proinflammatory substances lipopolysaccharide (LPS) and tumor necrosis factor (TNF). We found that the number of vesicles produced by adMSC does not change upon stimulation of the cells with LPS and TNF. Furthermore, adMSC-derived extracellular vesicles exert procoagulant activity independent of previous stimulation with LPS or TNF. We found evidence that the vesicles induce coagulation via both the intrinsic and the extrinsic pathway of coagulation.
细胞外囊泡由许多不同类型的细胞产生，其中包括不同来源的间充质基质/干细胞 (MSC)。已经表明，MSC 的细胞外囊泡发挥与细胞本身相似的治疗作用。在这里，我们分离并鉴定了脂肪来源的 MSC（adMSC）在受到促炎物质脂多糖（LPS）和肿瘤坏死因子（TNF）刺激时在体外产生的细胞外囊泡。我们发现，当用 LPS 和 TNF 刺激细胞时，产生的囊泡数量不会改变。此外，adMSC 衍生的细胞外囊泡具有独立于先前用 LPS 或 TNF 刺激的促凝活性。我们发现证据表明，囊泡通过凝血的内在和外在途径诱导凝血。
