Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal; Current address: International Iberian Nanotechnology Laboratory (INL), Braga, Portugal.
Trends Biotechnol. 2018 Sep;36(9):966-984. doi: 10.1016/j.tibtech.2018.04.006. Epub 2018 May 16.
Bacteriophages encode many distinct proteins for the successful infection of a bacterial host. Each protein plays a specific role in the phage replication cycle, from host recognition, through takeover of the host machinery, and up to cell lysis for progeny release. As the roles of these proteins are being revealed, more biotechnological applications can be anticipated. Phage-encoded proteins are now being explored for the control, detection, and typing of bacteria; as vehicles for drug delivery; and for vaccine development. In this review, we discuss how engineering approaches can be used to improve the natural properties of these proteins and set forth the most innovative applications that demonstrate the unlimited biotechnological potential held by phage-encoded proteins.
噬菌体编码许多不同的蛋白质，以成功感染细菌宿主。每种蛋白质在噬菌体复制周期中都发挥着特定的作用，从宿主识别、接管宿主机制，到细胞裂解以释放子代。随着这些蛋白质作用的揭示，可以预期更多的生物技术应用。噬菌体编码的蛋白质现在正被探索用于细菌的控制、检测和分型；作为药物输送的载体；以及疫苗开发。在这篇综述中，我们讨论了如何利用工程方法来改善这些蛋白质的天然特性，并提出了最具创新性的应用，展示了噬菌体编码蛋白质所具有的无限生物技术潜力。
