Buravkova L B, Andreeva E R, Lobanova M V, Cotnezova E V, Grigoriev A I
Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, 123007, Russia.
Dokl Biochem Biophys. 2018 Mar;479(1):69-71. doi: 10.1134/S1607672918020047. Epub 2018 May 19.
The dynamics of the expression of genes encoding adhesion molecules, molecules of the connective tissue matrix, and its remodeling enzymes was studied in multipotent mesenchymal stromal cells (MSCs) from human adipose tissue after interaction with cord blood hematopoietic progenitors (HSPCs). An upregulation of ICAM1 and VCAM1, directly proportional to the coculture time (24-72 h), was found. After 72 h of culturing, a downregulation of the genes encoding the majority of matrix molecules (SPP1; COL6A2,7A1; MMP1,3; TIMP1,3; and HAS1) and cell-matrix adhesion molecules (ITGs) was revealed. The detected changes may ensure the realization of the stromal MSC function due to improvement of adhesion and transmigration of HSPCs into the subcellular space.
在人脂肪组织来源的多能间充质基质细胞（MSC）与脐血造血祖细胞（HSPC）相互作用后，对编码黏附分子、结缔组织基质分子及其重塑酶的基因表达动态进行了研究。发现ICAM1和VCAM1上调，且与共培养时间（24 - 72小时）成正比。培养72小时后，发现编码大多数基质分子（SPP1；COL6A2、7A1；MMP1、3；TIMP1、3；和HAS1）以及细胞 - 基质黏附分子（ITG）的基因下调。检测到的这些变化可能通过改善HSPC向亚细胞空间的黏附和迁移来确保间充质基质细胞功能的实现。
