Sotnezova E V, Andreeva E R, Grigoriev A I, Buravkova L B
Institute of Biomedical Problems of the Russian Academy of Sciences, Khoroshovskoye shosse 76A, Moscow, 123007, Russia.
Acta Naturae. 2016 Jul-Sep;8(3):6-16.
Transplantation of umbilical cord blood cells is currently widely used in modern cell therapy. However, the limited number of hematopoietic stem and progenitor cells (HSPCs) and prolonged time of recovery after the transplantation are significant limitations in the use of cord blood. expansion with various cytokine combinations is one of the most common approaches for increasing the number of HSPCs from one cord blood unit. In addition, there are protocols that enable amplification of cord blood cells based on native hematopoietic microenvironmental cues, including stromal components and the tissue-relevant oxygen level. The newest techniques for expansion of HSPCs are based on data from the elucidation of the molecular mechanisms governing the hematopoietic niche function. Application of these methods has provided an improvement of several important clinical outcomes. Alternative methods of cord blood transplantation enhancement based on optimization of HPSC homing and engraftment in patient tissues have also been successful. The goal of the present review is to analyze recent methodological approaches to cord blood HSPC amplification.
脐带血细胞移植目前在现代细胞治疗中被广泛应用。然而，造血干细胞和祖细胞（HSPCs）数量有限以及移植后恢复时间延长是脐带血使用中的重大限制。用各种细胞因子组合进行扩增是增加单个脐带血单位中HSPCs数量的最常用方法之一。此外，还有一些方案能够根据天然造血微环境线索（包括基质成分和组织相关氧水平）扩增脐带血细胞。HSPCs扩增的最新技术基于对造血微环境功能调控分子机制的阐明数据。这些方法的应用改善了几个重要的临床结果。基于优化HPSC在患者组织中的归巢和植入的脐带血移植增强替代方法也取得了成功。本综述的目的是分析脐带血HSPC扩增的最新方法。
