Dygalo N N, Kalinina T S, Lanshakov D A
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 6300900, Russia.
Novosibirsk State University, Novosibirsk, 630090, Russia.
Dokl Biochem Biophys. 2018 Mar;479(1):108-110. doi: 10.1134/S160767291802014X. Epub 2018 May 19.
Irradiation of a mixture of oligonucleotides with dextran resulted in the formation of a complex that is detected by a decelerated migration of an irradiated sample in electrophoretic gel compared to a non-irradiated one. When injected into the brain of neonatal rats, the formed complex penetrated into the cells 3 times easier compared to the original oligonucleotide, thus indicating that the use of radiation crosslinking of oligonucleotides with oligosaccharides is promising to enhance the efficiency of delivery of gene-targeted oligonucleotide drugs into cells.
用葡聚糖辐照寡核苷酸混合物会导致形成一种复合物，与未辐照的样品相比，辐照后的样品在电泳凝胶中的迁移速度减慢，从而检测到该复合物。当将形成的复合物注射到新生大鼠的大脑中时，与原始寡核苷酸相比，它穿透细胞的难度降低了3倍，这表明使用寡核苷酸与寡糖的辐射交联有望提高基因靶向寡核苷酸药物向细胞内递送的效率。
