College of veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
College of animal science and technology, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
Cell Stress Chaperones. 2018 Sep;23(5):1033-1040. doi: 10.1007/s12192-018-0912-3. Epub 2018 May 20.
The present study investigates the effects of heat stress on the kidney in broilers, based on previous findings which showed that heat stress caused cardiac damage in broilers. Further, the possible renoprotective role of aspirin and the heat shock proteins HSP60 and HSP47 was also investigated. The enzyme levels of urea and uric acid, which are indicators of renal damage, and lactate dehydrogenase, an indicator of oxidative damage, were measured in chickens that were only exposed to heat stress, chickens that were pretreated with aspirin before heat stress, and chickens that were only treated with aspirin. Further, histological examination of renal tissue from the three groups was also performed. Finally, expression of HSP60 and HSP47 was also examined. In the heat stress group, the enzyme measurements were indicative of renal dysfunction and oxidative damage, and the histological findings were indicative of renal ischemia and damage. Aspirin seemed to have a protective effect against the renal damage caused by the stress, based on the enzyme measurements and histopathological findings in the aspirin-treated group. The findings also indicate that aspirin may induce HSP60 and HSP47 expression in renal cells. Finally, the expression patterns of HSP60 and HSP47 indicated that they may play a renoprotective role, as their expression was higher in the aspirin-treated groups. In conclusion, the present findings show that heat stress causes renal damage in poultry and that aspirin may play a protective role against this damage via pathways that involve HSP60 and HSP47.
本研究基于先前的研究结果，即热应激会导致肉鸡心脏损伤，进一步探讨了热应激对肉鸡肾脏的影响。此外，还研究了阿司匹林和热休克蛋白 HSP60 和 HSP47 可能的肾保护作用。在仅暴露于热应激的鸡、在热应激前用阿司匹林预处理的鸡和仅用阿司匹林治疗的鸡中，测量了尿素和尿酸（肾损伤的指标）以及乳酸脱氢酶（氧化损伤的指标）的酶水平。进一步对三组鸡的肾组织进行了组织学检查。最后，还检查了 HSP60 和 HSP47 的表达。在热应激组中，酶测量结果表明肾功能和氧化损伤，组织学发现表明肾缺血和损伤。基于阿司匹林治疗组的酶测量和组织病理学发现，阿司匹林似乎对应激引起的肾脏损伤具有保护作用。研究结果还表明，阿司匹林可能诱导肾细胞中 HSP60 和 HSP47 的表达。最后，HSP60 和 HSP47 的表达模式表明它们可能发挥肾保护作用，因为它们在阿司匹林治疗组中的表达更高。总之，本研究结果表明，热应激会导致家禽肾脏损伤，而阿司匹林可能通过 HSP60 和 HSP47 途径发挥保护作用。
