重复使用NALC/NaOH去污对Xpert MTB/RIF检测性能的影响。

The impact of repeated NALC/NaOH- decontamination on the performance of Xpert MTB/RIF assay.

作者信息

Rachow Andrea, Saathoff Elmar, Mtafya Bariki, Mapamba Daniel, Mangu Chacha, Rojas-Ponce Gabriel, Ntinginya Nyanda E, Boeree Martin, Heinrich Norbert, Gillespie Stephen H, Hoelscher Michael

机构信息

Division of Infectious Diseases and Tropical Medicine, Medical Centre of the University of Munich (LMU), Munich, Germany; German Centre for Infection Research (DZIF), Partner Site Munich, Germany.

出版信息

Tuberculosis (Edinb). 2018 May;110:56-58. doi: 10.1016/j.tube.2018.04.001. Epub 2018 Apr 5.

DOI:10.1016/j.tube.2018.04.001

PMID:29779774

Abstract

The Xpert MTB/RIF assay detects Mycobacterium tuberculosis in unprocessed or NALC/NaOH- decontaminated sputum. The effect of repeated NALC/NaOH-decontamination on several Xpert performance parameters was assessed in this study. A second NALC/NaOH-decontamination had no effect on the binary Xpert-outcome but increased the value for the quantitative readout (C). Repeated decontamination was not associated with PCR-inhibition or invalid results. The C of M.tb positive samples was higher in inhibited Xpert runs. Our data indicate that NALC/NaOH-decontamination has an effect on the performance of the Xpert assay, and that C readouts of decontaminated sputum samples should be interpreted with caution.

摘要

Xpert MTB/RIF检测法可检测未经处理或经NALC/NaOH去污处理的痰液中的结核分枝杆菌。本研究评估了重复进行NALC/NaOH去污处理对Xpert多个性能参数的影响。第二次NALC/NaOH去污处理对Xpert的二元检测结果没有影响，但增加了定量读数（C）的值。重复去污处理与PCR抑制或无效结果无关。在Xpert检测结果受抑制的情况下，结核分枝杆菌阳性样本的C值更高。我们的数据表明，NALC/NaOH去污处理会影响Xpert检测法的性能，对去污处理后的痰液样本的C读数应谨慎解读。