Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
Int J Biol Macromol. 2018 Sep;116:1146-1152. doi: 10.1016/j.ijbiomac.2018.05.126. Epub 2018 May 18.
Versatile spider silk proteins have been prepared by various methods in morphology of spheres for functional applications. Inspired from natural spinning process, a facile approach for the fabrication of silk spheres is described. Distinct from the traditional emulsification method, silk spheres were assembled as rapidly as 10 s by using the HFIP-on-Oil method without any surfactants and agitation used. Notably, a series of factors, such as evaporation rate of HFIP, polarity and molecular weight of oils play central roles on the final silk morphologies. With regard to the increase of protein concentrations, the average dimension and size distribution of silk spheres were both increased. Together with present study, silk spheres prepared by other methods were summarized for comparison in drug delivery applications. As a proof-of-concept, silk spheres loaded with Rhodamine B and Doxorubicin were investigated for the potential proteinase-enhanced drug delivery. On the extracellular environment, ethanol-mediated silk spheres exhibited higher resistance against enzymatic degradation of proteinase K when compared with pristine spheres. Under fluorescent detection by the aid of CLSM, proteinase-enhanced release behaviors were further demonstrated through in-vitro experiments within Hela cells. The facile fabrication of spheres with tunable β-sheets establishes a fascinating platform for functional silk-based applications.
通过各种方法在球体形态上制备了多功能蜘蛛丝蛋白，以用于功能应用。受自然纺丝过程的启发，本文描述了一种用于制造丝球的简单方法。与传统的乳化方法不同，通过 HFIP-on-Oil 方法，在不使用任何表面活性剂和搅拌的情况下，可在 10 秒内迅速组装丝球。值得注意的是，一系列因素，如 HFIP 的蒸发率、油的极性和分子量，对最终的丝形态起着核心作用。随着蛋白质浓度的增加，丝球的平均尺寸和尺寸分布都增加了。结合本研究，还对通过其他方法制备的丝球在药物传递应用中的进行了综述和比较。作为概念验证，研究了载有 Rhodamine B 和阿霉素的丝球在潜在的蛋白酶增强药物传递中的应用。在细胞外环境中，与原始丝球相比，乙醇介导的丝球在蛋白酶 K 的酶降解方面表现出更高的抗性。通过共聚焦显微镜的荧光检测，进一步通过 HeLa 细胞内的体外实验证明了蛋白酶增强的释放行为。可调节β-折叠的球体的简单制造为基于丝的功能应用建立了一个引人入胜的平台。
