Martin N, Guiraud-Vitaux F, Colas-Linhart N, Bok B, Nahum H
Department of Neuroradiology, Hôpital Beaujon, Clichy, France.
Int J Rad Appl Instrum B. 1988;15(4):357-60. doi: 10.1016/0883-2897(88)90003-7.
Modifications in beta-endorphin levels in cerebrospinal fluid have been described following lumbar puncture and metrizamide injection. Cerebrospinal fluid (CSF) samples were obtained from 19 patients before and after lumbar myelography. Two radioimmunoassays were used. One was a commercial kit; and the other one (developed in our laboratory) used a chromatographic removal from beta-lipotrophin. No definite variation of beta-endorphin was observed after myelography, using either the commercial kit or a more sophisticated procedure. Some controls were prepared by adding metrizamide or Iopamidol in vitro to CSF samples in order to evaluate a non specific effect of these contrast media. The results obtained with these controls suggest that the discrepancy of results may be explained simply by assay artifacts due to drug interferences when using the commercial methods.
腰椎穿刺和注射甲泛葡胺后，脑脊液中β-内啡肽水平会发生改变。在19例患者进行腰椎脊髓造影前后采集脑脊液样本。使用了两种放射免疫测定法。一种是商业试剂盒；另一种（在我们实验室开发）是通过从β-促脂素进行色谱分离。使用商业试剂盒或更复杂的方法，脊髓造影后均未观察到β-内啡肽有明确变化。通过在体外向脑脊液样本中添加甲泛葡胺或碘帕醇制备了一些对照，以评估这些造影剂的非特异性作用。这些对照所获得的结果表明，使用商业方法时，结果差异可能仅仅是由于药物干扰导致的测定假象所致。
