Department of Food, Agriculture and Bioresources, School of Environment, Resources and Development, Asian Institute of Technology, 58 Moo 9, Km. 42, Paholyothin Highway, Klong Luang, Pathumthani 12120, Thailand.
Department of Food, Agriculture and Bioresources, School of Environment, Resources and Development, Asian Institute of Technology, 58 Moo 9, Km. 42, Paholyothin Highway, Klong Luang, Pathumthani 12120, Thailand.
Talanta. 2018 Aug 15;186:286-292. doi: 10.1016/j.talanta.2018.04.073. Epub 2018 Apr 25.
The aim of this work was to develop a programmable flow system for rapid assessment of total antioxidant capacity (TAC). Novel features of the prototype include a single pressure-driven source, versatile manipulation of fluid flows, ability to adapt to different TAC assays, and compatibility with microfluidic design. Antioxidant activity was determined by electrochemical measurement of residual 2,2-diphenyl-2-picrylhydrayl hydrate (DPPH•) free radicals in the solution. The overall performance of the device was validated by the spectrophotometric method. The results indicated that dosing of reagents and samples could be controlled by pressure (R = 0.992) and time (R = 0.999) with high accuracy, and the mixing uniformity of the device was equivalent to that of the batch mixing (R = 0.994). TAC assays of a standard antioxidant, ascorbic acid, as well as selected samples such as orange and pomegranate juices, white wine, and green tea by the device were comparable to traditional measurement techniques. Due to the short incubation time, the approach may be more suitable for fast, rather than slow reacting antioxidant compounds. The developed system could be used for rapid TAC screening of food and biological samples.
本工作旨在开发一种用于快速评估总抗氧化能力 (TAC) 的可编程流动系统。该原型的新颖特点包括单一压力驱动源、灵活的流体操控、适应不同 TAC 测定法的能力以及与微流控设计的兼容性。通过电化学测量溶液中残留的 2,2-二苯基-1-苦肼基自由基 (DPPH•) 来确定抗氧化活性。该设备的整体性能通过分光光度法进行了验证。结果表明，试剂和样品的加样可以通过压力 (R = 0.992) 和时间 (R = 0.999) 进行高精度控制，并且该设备的混合均匀度与批处理混合相当 (R = 0.994)。通过该设备对标准抗氧化剂抗坏血酸以及橙汁、石榴汁、白葡萄酒和绿茶等选定样品进行的 TAC 测定与传统测量技术相当。由于孵育时间短，该方法可能更适合快速而非缓慢反应的抗氧化化合物。所开发的系统可用于快速筛选食品和生物样品中的 TAC。
