Dipartimento di Agraria and Unità di Ricerca Istituto Nazionale di Biostrutture e Biosistemi, Università degli Studi di Sassari, Viale Italia 39, I-07100 Sassari, Italy.
Dipartimento di Medicina Clinica e Sperimentale, Università degli Studi di Sassari, Viale S. Pietro 43/b, I-07100 Sassari, Italy.
Talanta. 2018 Aug 15;186:53-59. doi: 10.1016/j.talanta.2018.04.034. Epub 2018 Apr 10.
Interference rejection in amperometric biosensors can be more effective introducing some modifiers during electro-deposition of permselective film. Addition of β-cyclodextrin (βCD), a cyclic oligosaccharide composed of seven glucose units, to the ortho-phenylendiamine (oPD) monomer were already demonstrated to provide an enhancement in ascorbic acid (AA) rejection. Here we evaluated the improvement in permselectivity of poly-eugenol and poly-magnolol films electro-polymerized in presence of different amounts of βCD or eugenol-βCD inclusion complex for amperometric biosensor application. Starting from Pt-Ir wire as transducer several microsensors were covered with polymeric films doped with βCD-based modifiers through constant potential amperometry. Characterization of modified polymers was achieved by scanning electron microscopy and permselectivity analysis. Poly-magnolol film in combination with βCD showed a worsening in permselectivity compared to poly-magnolol alone. In contrast, the introduction of βCD-based modifier enhanced the interference rejection toward the archetypal interferent AA, while slightly affecting permeability toward HO compared to the poly-eugenol without modifier. The AA rejection seems to be influenced by the availability of βCD cavity as well as film performance due to concentration of βCD-Eugenol inclusion complex. A poly-eugenol film co-polymerized with 2 mM βCD-eugenol inclusion complex showed a permselectivity equal to poly-orthophenylendiamine film (PPD), with a lower permeability to AA, likely to be related with a self-blocking mechanism. Based on these results, a biosensor for glutamate was constructed with a poly-eugenol doped with βCD-eugenol as permselective layer and its permselectivity, stability and lifetime were determined.
在电沉积选择透过性膜的过程中引入一些修饰剂，可以更有效地抑制安培生物传感器中的干扰。在邻苯二胺（oPD）单体中添加β-环糊精（βCD），一种由七个葡萄糖单元组成的环状低聚糖，已被证明可以提高抗坏血酸（AA）的排斥率。在这里，我们评估了在不同量的βCD 或丁香基-βCD 包合物存在下电聚合的聚丁香酚和聚厚朴酚膜的对映体选择性的改善，用于安培生物传感器应用。从 Pt-Ir 丝作为换能器开始，通过恒电位安培法在几个微传感器上覆盖了掺杂有基于βCD 的修饰剂的聚合物薄膜。通过扫描电子显微镜和对映体选择性分析对改性聚合物进行了表征。与单独的聚厚朴酚相比，聚厚朴酚与βCD 结合的膜的对映体选择性变差。相比之下，引入基于βCD 的修饰剂增强了对典型干扰物 AA 的干扰排斥作用，同时与没有修饰剂的聚丁香酚相比，对 HO 的渗透性略有影响。AA 的排斥似乎受到βCD 空腔的可用性以及由于βCD-丁香酚包合物浓度而导致的膜性能的影响。与聚邻苯二胺（PPD）膜相比，共聚合了 2mM βCD-丁香酚包合物的聚丁香酚膜表现出相等的对映体选择性，对 AA 的渗透性较低，这可能与自阻断机制有关。基于这些结果，构建了一种基于聚丁香酚掺杂βCD-丁香酚作为选择透过性层的谷氨酸生物传感器，并测定了其对映体选择性、稳定性和寿命。
