Hryn Volodymyr H, Deineha Tamara F, Piliuhin Аndrij V, Lavrenko Anna V, Lavrenko Dmytro O
Higher State Educational Establishment Of Ukraine «Ukrainian Medical Stomatological Academy», Poltava, Ukraine.
Wiad Lek. 2018;71(2 pt 2):303-306.
Introduction: Salivary glands have a significant impact on the state of the homeostasis of the human body, oral cavity in particular, sensitively responding to pathological processes. The reactivity of the salivary glands in response to pathological processes that are organically linked to morphology and functions of the organ's structures, and particularly the excretory ducts of the glands and their microcirculatory blood flow, is one of the problems which have not been solved to date. The aim of the paper was to elucidate the features of the stereomicroscopic structure of the excretory ducts and sectors of the microcirculatory blood flow in labial glands of older adults.
Materials and methods: The object of the study was the labial mucosa of the older adults, which was cut into 3х3 mm pieces and fixed in the buffered 4% glutaraldehyde solution with subsequent 2-hour fixation in osmium. Once the pieces were washed and dehydrated they were embedded into the Epon-812. The series of the semi-thin sections, made from the obtained epoxy blocks, were stained in phosphate buffered 0,1% toluidine blue solution. The serial semi-thin sections were subjected to histological and cytological studies and multilayered plastic reconstruction.
Results and conclusions: The series of histological epoxy semi-thin sections, as well as graphic and plastic reconstruction of the sectors of microcirculatory blood flow and excretory ducts of the human labial glands have demonstrated a range of morphological facts that can be used to clarify the intertissue stereological relationships. They also determined the syntopic proximity of the capacitive sectors of microcirculatory blood flow to the excretory ducts of the gland. Such pattern is especially notable in the collecting venules and intralobular ducts. It has been shown that the biggest venule is the collecting venous vessel. Anastomoses between the intralobular arterioles and collecting venules have been found in the microcirculatory blood flow of the labial glands.
引言：唾液腺对人体的内环境稳态状态有重大影响，尤其是口腔，能对病理过程做出敏感反应。唾液腺对与器官结构的形态和功能有机相关的病理过程的反应性，特别是腺体的排泄导管及其微循环血流，是迄今为止尚未解决的问题之一。本文旨在阐明老年人唇腺排泄导管和微循环血流区域的立体显微镜结构特征。
材料与方法：研究对象为老年人的唇黏膜，将其切成3×3毫米的小块，固定于4%的戊二醛缓冲溶液中，随后用锇固定2小时。小块经冲洗和脱水后，包埋于Epon - 812中。从所得环氧块制作的一系列半薄切片，用磷酸盐缓冲的0.1%甲苯胺蓝溶液染色。对系列半薄切片进行组织学和细胞学研究以及多层塑料重建。
结果与结论：一系列组织学环氧半薄切片，以及人体唇腺微循环血流区域和排泄导管的图形及塑料重建，都显示出一系列形态学事实，可用于阐明组织间的立体关系。它们还确定了微循环血流的电容性区域与腺体排泄导管的同位接近度。这种模式在集合小静脉和小叶内导管中尤为明显。已表明最大静脉是集合静脉血管。在唇腺的微循环血流中发现了小叶内小动脉与集合小静脉之间的吻合。
