Buck F, Hahn K D, Brill W, Rüterjans H, Chernov B K, Skryabin K G, Kirpichnikov M P, Bayev A A
Institut für Biophysikalische Chemie, Universität Frankfurt, FRG.
J Biomol Struct Dyn. 1986 Apr;3(5):899-911. doi: 10.1080/07391102.1986.10508472.
The phage lambda operator OR1 and a 18 base pair symmetric lac operator have been studied by high resolution NMR. The imino proton resonances and the resonances of the unexchangeable protons (except the 5' and 5" sugar proton resonances) have been assigned by one- and two-dimensional NOE techniques. The imino proton resonances of OR1 and the symmetric lac operator have been used to monitor changes induced in the operator structure by the formation of a specific complex with the phage lambda cro protein and with the lac repressor N-terminal DNA binding domain ("headpiece"). Two regions within the OR1 sequence could be identified, where changes in the imino proton resonance positions occur: The central part around base pairs CG 9 and 10 and the region around base pairs AT 5 and CG 5. The TA base pair 6 is the only position in the symmetric lac operator, where the complex formation with headpiece induces a change.
噬菌体λ操纵子OR1和一个18个碱基对的对称lac操纵子已通过高分辨率核磁共振进行了研究。亚氨基质子共振以及不可交换质子（除5'和5''糖质子共振外）的共振已通过一维和二维NOE技术进行了归属。OR1和对称lac操纵子的亚氨基质子共振已被用于监测与噬菌体λ cro蛋白和lac阻遏物N端DNA结合结构域（“头部”）形成特异性复合物时操纵子结构中诱导的变化。在OR1序列内可识别出两个区域，其中亚氨基质子共振位置发生变化：碱基对CG 9和10周围的中心部分以及碱基对AT 5和CG 5周围的区域。TA碱基对6是对称lac操纵子中唯一与头部形成复合物时诱导变化的位置。
